Background: Caenorhabditis elegans is a widely used model animal. Chemotaxis assay is one of the experiments that study the effects of different chemicals on nematodes. It is mainly used to study the effects of different chemicals on the perception behavior of nematodes. By conducting this experiment, not only can the neurotoxicity of chemicals be reflected, but also the impact of chemicals on physiological functions regulated by the nervous system, such as nematode feeding behavior and basic motor ability.
Objective: The experiment of detecting the response of nematode to chemicals is also a common method of chemical toxicity testing based on nematode models. In the analysis of worm tendency behavior, manual operations are generally used. Manually processing a large number of worms under a microscope is very time-consuming and labor-intensive. The current quantitative methods for nematode chemotaxis experiments are not only time-consuming and labor-intensive, but also biased in experimental results due to differences in judgment standards among experimenters. The automatic and efficient quantification method for nematode chemotaxis experiments is a very important technical difficulty in the field of nematode experiments.
Methods: Here, we have designed an automatic quantification method for nematode chemotaxis experiments by incorporating image acquisition and processing techniques into the nematode experiment.
Results: The experimental results show that the Pearson correlation coefficient between manual and automatic counting results is 0.978.
Conclusion: This proves the effectiveness of our method. Applying the automatic measurement method to replace manual counting by the experimenter can improve work efficiency, and reduce errors in human counting operations.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11191428 | PMC |
| http://dx.doi.org/10.3233/THC-248013 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Caenorhabditis Elegans as a Model for Environmental Epigenetics.
Curr Environ Health Rep
January 2025
Institute for Society and Genetics, University of California, Boyer Hall, Room 332, 611 Charles E Young Dr E., UCLA, Los Angeles, CA, 90095, USA.
Purpose Of Review: The burgeoning field of environmental epigenetics has revealed the malleability of the epigenome and uncovered numerous instances of its sensitivity to environmental influences; however, pinpointing specific mechanisms that tie together environmental triggers, epigenetic pathways, and organismal responses has proven difficult. This article describes how Caenorhabditis elegans can fill this gap, serving as a useful model for the discovery of molecular epigenetic mechanisms that are conserved in humans.
Recent Findings: Recent results show that environmental stressors such as methylmercury, arsenite, starvation, heat, bacterial infection, and mitochondrial inhibitors can all have profound effects on the epigenome, with some insults showing epigenetic and organismal effects for multiple generations.
Bioactive Secondary Metabolites from Against .
Microorganisms
December 2024
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Life Sciences, Yunnan University, Kunming 650091, China.
Root-knot nematodes (RKNs) are pathogens that endanger a wide range of crops and cause serious global agricultural losses. In this study, we investigated metabolites of the endoparasitic fungus YMF1.01751, with the expectation of discovering valuable biocontrol compounds.
View Article and Find Full Text PDFCarbon dioxide shapes parasite-host interactions in a human-infective nematode.
Curr Biol
December 2024
Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095, USA; Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address:
Article Synopsis
- Skin-penetrating nematodes, like Strongyloides stercoralis, infect nearly one billion people by penetrating skin and developing into adults in the host's small intestine.
- Researchers investigated how these nematodes use carbon dioxide (CO) to find hosts and navigate within them, revealing that the larvae exhibit different responses to CO depending on their life stage.
- Specific sensory neurons and the receptor Ss-GCY-9 are crucial for these CO-evoked behaviors, providing insights that could lead to new treatments targeting the nematodes' chemosensory pathways.
The chemotactic response of Caenorhabditis elegans represents a promising tool for the early detection of cancer.
Discov Oncol
December 2024
Department of Laboratory Medicine, Faculty of Health Care and Social Work, Trnava University in Trnava, Univerzitné Námestie 1, 91843, Trnava, Slovakia.
Article Synopsis
- Caenorhabditis elegans is being explored as a tool for chemotaxis testing due to its sensitive olfactory system, especially in cancer diagnostics.
- This study aimed to assess the nematode's ability to differentiate between urine samples from healthy individuals and those with breast or colon cancer.
- Results indicated a significant difference in the nematodes' responses, with high diagnostic sensitivity (96% for breast cancer, 100% for colon cancer) and moderate specificity (62%) in detecting cancer through urinary volatile organic compounds.
Evaluation of N-NOSE as a surveillance tool for recurrence in gastric and esophageal cancers: a prospective cohort study.
BMC Cancer
December 2024
Department of Gastrointestinal Surgery, Graduate School of Medicine, The University of Tokyo, Tokyo, 113-8655, Japan.
Objective: Early detection of recurrent gastric and esophageal cancers remains a critical challenge. Innovative and non-invasive cancer screening technologies, such as N-NOSE, can improve early detection. N-NOSE is a urine-based scent test that leverages the olfactory abilities of the nematode C.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!