AI Article Synopsis
- The study aimed to investigate if orangutans at a Wisconsin zoo had antimicrobial-resistant bacteria, linking them to human strains due to close contact with zoo staff.
- Fecal samples from two orangutans were tested for resistant bacteria using specific culture media, and identification was done through mass spectrometry and antimicrobial testing.
- One bacterial isolate was further analyzed and identified as Escherichia coli, specifically the ST6448 strain with a resistance gene (CTX-M-55), suggesting a potential risk of resistance transfer from animals to humans.
Article Abstract
The purpose of this study was to determine if orangutans (Pongo spp.) living in captivity at a zoo in Wisconsin were colonized with antimicrobial-resistant bacteria and, if found, to identify underlying genetic mechanisms contributing to their resistant phenotypes. We hypothesize that since antimicrobial-resistant bacteria are so prevalent within humans, the animals could also be carriers of such strains given the daily contact between the animals and the zoo staff that care for them. To test this theory, fecal samples from two orangutans were examined for resistant bacteria by inoculation on HardyCHROM™ ESBL and HardyCHROM™ CRE agars. Isolates were identified using MALDI-TOF mass spectrometry and antimicrobial susceptibility testing was performed using a Microscan autoSCAN-4 System. An isolate was selected for additional characterization, including whole genome sequencing (WGS). Using the Type (Strain) Genome Server (TYGS) the bacterium was identified as Escherichia coli. The sequence type identified was (ST/phylogenetic group/β-lactamase): ST6448/B1/CTX-M-55.
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| http://dx.doi.org/10.1007/s00284-024-03693-x | DOI Listing |
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