Introduction: Quercetin (Qc), rutin (Ru), and hyperoside (Hyp) are three common polyphenols widely distributed in the plant kingdom.
Method: This study explored the inhibition and mechanisms of Qc, Ru, and Hyp against xanthine oxidase (XOD) by enzyme kinetic analysis, fluorescence analysis, and molecular docking. The inhibitory activities of the three polyphenols on XOD showed the following trend: quercetin > hyperoside > rutin, with IC50 values of 8.327 ± 0.36 µmol/L, 35.215 ± 0.4 µmol/L and 60.811 ± 0.19 µmol/L, respectively. All three polyphenols inhibited xanthine oxidase activity in a mixed-competitive manner. Synchronous fluorescence results demonstrated that three polyphenols binding to XOD were spontaneous and showed static quenching.
Result: The binding of the three polyphenols to XOD is mainly driven by hydrogen bonding and van der Waals forces, resulting in the formation of an XOD-XA complex with only one affinity binding site. The binding sites of the three RSFQ phenolic compounds are close to those of tryptophan. Molecular docking showed that all three polyphenols enter the active pocket of XOD and maintain the stability of the complex through hydrogen bonding, hydrophobic interaction, and van der Waals forces.
Conclusion: The results provide a theoretical basis for quercetin, rutin, and hyperoside to be used as function factors to prevent hyperuricemia.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2174/0113892010297269240427055003 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Variation in Nutritional Quality of By-Products from Nine Organic Apple Cultivars.
Food Technol Biotechnol
December 2024
Faculty of Biotechnology, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Marasti Blvd., Bucharest, Romania.
Research Background: Recently, consumers have been increasingly interested in highly nutritional and health-promoting products in the form of functional foods that are produced using environmentally friendly processes as part of the circular economy. Therefore, much research has been carried out related to the valorisation of waste generated during the processing of food, especially fruit and vegetables, commonly referred to as by-products. These by-products consist of peels, seeds, stems or pomace, which have been shown to have valuable nutritional properties (high content of polyphenols, vitamins, antioxidants, ).
View Article and Find Full Text PDFEffects of Resveratrol Supplementation on Delayed Onset Muscle Soreness and Muscle Recovery: A Systematic Review.
Malays J Med Sci
December 2024
Department of Biomedical Imaging, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Pulau Pinang, Malaysia.
Delayed onset muscle soreness (DOMS) and impaired muscle recovery significantly affect athletes and recreational exercisers, influencing their performance and training consistency. Resveratrol, a natural polyphenol known for its anti-inflammatory and antioxidant properties, is thought to mitigate these effects, yet its effectiveness remains to be fully verified. This systematic review evaluates the impact of RES supplementation on muscle recovery in adults by examining its influence on DOMS, oxidative stress, and inflammation, along with its interactions with other supplements.
View Article and Find Full Text PDFExploring the therapeutic potential of acorn extract in papillomavirus-induced lesions.
Vet World
November 2024
Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal.
Background And Aim: Papillomaviruses (PVs) infections have been documented in numerous animal species across different regions worldwide. They often exert significant impacts on animal health and livestock production. Scientists have studied natural products for over half a century due to their diverse chemical composition, acknowledging their value in fighting cancer.
View Article and Find Full Text PDFToona sinensis fruit polyphenols alleviate cerebral ischemia-reperfusion injury in rats by inhibiting MAPK signaling pathways and NLRP3 inflammasome/pyroptosis.
J Ethnopharmacol
January 2025
Department of Pharmacology, College of Medicine, Jiaxing University, Jiaxing, 314001, PR China; Third-grade Pharmacological Laboratory on Traditional Chinese Medicine, State Administration of Traditional Chinese Medicine, China Three Gorges University, Yichang 443002, PR China; College of Basic Medical Sciences, China Three Gorges University, Yichang 443002, PR China. Electronic address:
Ethnopharmacological Relevance: Toona sinensis fruit polyphenols (TSFP) are polyphenols that have been separated and extracted from mature Toona sinensis fruits. TSFP anti-inflammatory and neuroprotective properties have demonstrated promise. However, the underlying mechanisms require more elucidation.
View Article and Find Full Text PDFPolyphenol-modified 3D Nanoassemblies: A novel antibacterial immunoglobulins loading platform for rapid detection of Salmonella typhimurium.
Food Chem
January 2025
College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road Yangling, 712100, Shaanxi, China; College of Food Engineering, Ludong University, Yantai, 264025, Shandong, China. Electronic address:
Within the realm of lateral flow assay (LFIA), the conjugation efficiency between signal tracers and antibody constitutes a pivotal determinant for the sensitivity of the detection system. In this study, three-dimensional (3D) complex flower-like MoS self-assembled from 2D MoS, and natural plant polyphenols "Tannic acid" were introduced for surface modification. This composite material exhibits distinct colorimetric signals, excellent monoclonal antibody coupling efficiency, and commendable photothermal properties.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!