Two novel species associated with bleeding cankers of broadleaf hosts, sp. nov. and sp. nov.

Seventeen Gram-negative, facultatively anaerobic bacterial strains were isolated from bleeding cankers of various broadleaf hosts and oak rhizosphere soil in Great Britain. The strains were tentatively identified as belonging to the genus based on 16S rRNA gene sequencing. Multilocus sequence analysis (MLSA), based on four protein-encoding genes (, , , and ), separated the strains into three clusters within the genus clade. The majority of strains clustered with the type strain of , with the remaining strains divided into two clusters with no known type strain. Whole genome sequencing comparisons confirmed these two clusters of strains as belonging to two novel species which can be differentiated phenotypically from their current closest phylogenetic relatives. Therefore, two novel species are proposed: sp. nov. (type strain = BAC 10a-01-01 = LMG 33072 = CCUG 77096) and sp. nov. (type strain = TW_WC1a.1 = LMG 33073 = CCUG 77094).