Assessment of Antibacterial Properties and Cytotoxic Effect of Ethanolic Extracts of Clitoria ternatea and Camellia sinensis Herbal Formulation Against Clinical Pathogens.

Aim The study aims to evaluate the antibacterial properties of ethanolic extracts from and against pathogens causing UTI, wound pathogens, and other clinical bacterial infections and their cytotoxic effects using the brine shrimp lethality assay (BSLA). Methods Ethanolic extracts of and were prepared, and their antibacterial activity was tested against , , , , and using the well diffusion method. The cytotoxicity was assessed through the BSLA, determining the LC values for each extract. Results The formulation of both plant extracts exhibited significant antibacterial activity against UTI pathogens, and wound pathogen bacteria showed higher efficacy compared to other studies. The BSLA revealed a dose-dependent increase in toxicity, with extracts demonstrating higher cytotoxicity than . Conclusion The ethanolic extracts of and possess antibacterial properties against UTI-causing bacteria and show cytotoxic effects in a brine shrimp model. These findings suggest the potential of these plants for developing alternative treatments for UTI. However, further research is necessary to fully understand their safety and efficacy in human subjects.