AI Article Synopsis

  • Previous research indicates that the type VI secretion system (T6SS) is crucial for competition and virulence in many gram-negative bacteria, though its role remains debated in certain strains.
  • The study focused on the PccS1 strain, identifying a cluster of 17 core T6SS genes and analyzing over 220 derivatives to categorize VgrGs and their interactions, which were vital for virulence and competition.
  • Findings revealed that proteins like FhaB were secreted in a redundant manner through T6SS and type IV secretion system (T4SS), highlighting a unique mechanism in bacterial effector secretion affecting virulence and host interactions.

Article Abstract

Previous studies revealed that the type VI secretion system (T6SS) has an essential role in bacterial competition and virulence in many gram-negative bacteria. However, the role of T6SS in virulence in remains controversial. We examined a closely related strain, PccS1, and discovered that its T6SS comprises a single-copy cluster of 17 core genes with a higher identity to homologs from Through extensive phenotypic and functional analyses of over 220 derivatives of PccS1, we found that three of the five VgrGs could be classified into group I VgrGs. These VgrGs interacted with corresponding DUF4123 domain proteins, which were secreted outside of the membrane and were dependent on either the T6SS or type IV secretion system (T4SS). This interaction directly governed virulence and competition. Meanwhile, supernatant proteomic analyses with strains defective in the T6SS and/or T4SS confirmed that effectors, such as FhaB, were secreted redundantly to control the virulence and suppress host callose deposition in the course of infection. Notably, this redundant secretion mechanism between the T6SS and T4SS is believed to be the first of its kind in bacteria.

Download full-text PDF

Source
http://dx.doi.org/10.1094/PHYTO-11-23-0455-RDOI Listing

Publication Analysis

Top Keywords

t6ss t4ss
8
bacterial competition
8
type secretion
8
secretion system
8
t6ss
7
virulence
5
t4ss redundantly
4
redundantly secrete
4
secrete effectors
4
effectors govern
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!