AI Article Synopsis
- 3D models are crucial in preclinical drug testing, accurately simulating cancer cell interactions with the tumor microenvironment.
- A detailed protocol is provided for creating scaffold-free 3D human melanoma spheroids, including characterization methods like live-cell imaging and histology.
- The models allow drug testing and assessment of cell death using various techniques, and can be adapted for co-cultures with immune cells.
Article Abstract
Three-dimensional (3D) models play an increasingly important role in preclinical drug testing as they faithfully mimic interactions between cancer cells and the tumor microenvironment (TME). Here, we present a protocol for generating scaffold-free 3D multicomponent human melanoma spheroids. We describe steps for characterizing models using live-cell imaging and histology, followed by drug testing and assessment of cell death through various techniques such as imaging, luminescence-based assays, and flow cytometry. Finally, we demonstrate the models' adaptability for co-cultures with immune cells.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11109877 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2024.103058 | DOI Listing |
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