Drought, as a primary environmental factor, imposes significant constraints on developmental processes and productivity of plants. PHDs were identified as stress-responsive genes in a wide range of eukaryotes. However, the regulatory mechanisms governing PHD genes in maize under abiotic stress conditions are still largely unknown and require further investigation. Here, we identified a mutant, zmvil2, in the EMS mutant library with a C to T mutation in the exon of the Zm00001d053875 (VIN3-like protein 2, ZmVIL2), resulting in premature termination of protein coding. ZmVIL2 belongs to PHD protein family. Compared to WT, zmvil2 mutant exhibited increased sensitivity to drought stress. Consistently, overexpression of ZmVIL2 enhances drought resistance in maize. Y2H, BiFC, and Co-IP experiments revealed that ZmVIL2 directly interacts with ZmFIP37 (FKBP12-interacting protein of 37). zmfip37 knockout mutants also exhibit decreased drought tolerance. Interestingly, we demonstrated that ZmABF4 directly binds to the ZmVIL2 promoter to enhance its activity in yeast one hybrid (Y1H), electrophoretic mobility shift assay (EMSA) and dual luciferase reporter assays. Therefore, we uncovered a novel model ZmABF4-ZmVIL2/ZmFIP37 that promotes drought tolerance in maize. Overall, these findings have enriched the knowledge of the functions of PHD genes in maize and provides genetic resources for breeding stress-tolerant maize varieties.
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http://dx.doi.org/10.1111/pce.14954 | DOI Listing |
Int J Biol Macromol
December 2024
Institute of Cotton, Hebei Academy of Agriculture and Forestry Sciences, Key Laboratory of Cotton Biology and Genetic breeding in Huanghuaihai Semiarid Area, Ministry of Agriculture and Rural Affairs, Shijiazhuang 050051, Hebei, China. Electronic address:
Abiotic stress poses adverse impacts on cotton production, raising demands for a better understanding of stress-response mechanisms and developing strategies to improve plant performance to cope with stress. CYSTM (Cysteine-rich transmembrane module) is a widely distributed and conserved family in eukaryotes that performs potential functions in stress tolerance. However, CYSTM genes and their role in stress response is uncharacterized in cotton.
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College of Forestry and Grasslands, Jilin Provincial Key Laboratory of Tree and Grass Genetics and Breeding, Jilin Agriculture University, Changchun 130118, China. Electronic address:
Background: Trehalose is a nonreducing disaccharide containing two glucose molecules linked through an α,α-1,1-glycosidic bond. This unique chemical structure causes trehalose levels to fluctuate significantly in plants under stress, where it functions as an osmoprotectant, enhancing plant resistance to stress. Previous studies have confirmed that the trehalose synthesis pathway is widely conserved across most plants.
View Article and Find Full Text PDFInt J Biol Macromol
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College of Horticulture, Hebei Agricultural University, Baoding, Hebei 071001, China; Research Center of Chinese Jujube, Hebei Agricultural University, Baoding, Hebei 071001, China. Electronic address:
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College of Enology, Northwest A & F University, Yangling, 712100, Shaanxi, China. Electronic address:
As a new plant hormone, strigolactone not only promotes leaf senescence, inhibits plant branching and regulates root structure, but also plays an important role in abiotic stress resistance. However, little is known about the function of VvCCD7 under abiotic stress, a key gene for the synthesis of strigolactone in grapevine. In this study, VvCCD7 gene was cloned from grape leaves of 'Cabernet Sauvignon'.
View Article and Find Full Text PDFJ Plant Physiol
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Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, College of Horticulture & Landscape Architecture, Northeast Agricultural University, Harbin, 150030, China; National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions, Northeast Agricultural University, Harbin, 150030, China. Electronic address:
MYB transcription factors exert crucial functions in enhancing plant stress tolerance, which is impacted by soil drought and salinity. In our study, the R2R3-type MYB transcription factor gene LcMYB5 from blue honeysuckle (Lonicera caerulea L.) was successfully cloned and identified, and confirmed its nuclear localization.
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