AI Article Synopsis
- * Through cloning and transgenic techniques involving Agrobacterium tumefaciens, researchers created a transgenic line to assess growth and physiological traits.
- * Results indicated that the transgenic plants exhibited faster growth, advanced flowering and fruiting, and changes in biochemical markers, linking the transcription factor's expression to these beneficial traits.
Article Abstract
The purpose of this study was to analyze the role of transcription factor in , proving that the transcription factor was related to the plant phenotypes of - cv. 'GuangYaoDa1' and it could be used in molecular-assisted breeding. 'GuangYaoDa1' was used as the material and its DNA was the template to clone DsWRKY6, the transgenic line was constructed by agrobacterium tumefaciens‑mediated transformation. Transgenic was cultivated to study phenotype and physiological and biochemical indexes. Phenotypic observation showed that transgenic had a faster growth rate while compared with the control group, they had longer lengths of main stem, lateral branches of cauline leaves, and root, but a lower number of cauline leaves and lateral branches of cauline leaves. And it also showed that their flowering and fruiting periods were advanced. The results of physiological and biochemical indexes showed that the relative expressions of increased and the abscisic acid content significantly increased in transgenic compared with the control group. According to the above results, could regulate the advancing of flowering and fruiting periods caused by the improvement of abscisic acid content, and expression of the transcription factor might be the cause of the upright growth of 'GuangYaoDa1'.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11095563 | PMC |
| http://dx.doi.org/10.1080/15592324.2024.2349868 | DOI Listing |
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