Effects of tumor necrosis factor- and interleukin- genetic variants on type 2 diabetes mellitus susceptibility and metabolic parameters among Jordanians.

Objectives: Diabetes mellitus (DM) is a complex chronic illness with diverse pathogenesis and associations with health complications. Genetic factors significantly contribute to DM development, and tumor necrosis factor alpha () and interleukin-10 () genes play major roles. This study aims to explore the influence of and genetic variants on T2DM development in Jordanian patients at Jordan University Hospital.

Methods: One-hundred and 60 diabetic and 159 non-diabetic subjects were genotyped for . Additionally, 181 diabetic and 191 non-diabetic subjects were genotyped for using PCR-RFLP genotyping method. The demographic, lipid, and glycemic parameters of the patients were obtained from the computer records in the hospital.

Results: and genetic variants exhibited significant different frequencies in non-T2DM subjects and T2DM patients. The difference in genotype frequency between non-T2DM and T2DM participants was significant under the dominant model, while the genotype frequency was significant under the recessive model. A significant association (p<0.05) was observed between and total cholesterol levels, and between polymorphism and glycosylated hemoglobin (HbA) and creatinine levels among T2DM patients.

Conclusions: and are identified as genetic risk factors for T2DM. These variants also correlate with variations in cholesterol, HbA, and creatinine levels among T2DM patients. Larger clinical studies are warranted to validate these findings.