Neuron-derived neurotrophic factor promotes the differentiation of intramuscular and subcutaneous adipocytes in goat.

Adipocyte play an important role in human health and meat quality by influencing the tenderness, flavor, and juiciness of mutton It has been shown that neuron-derived neurotrophic factor () is closely related to energy metabolism and adipocyte differentiation in bovine. However, the role of in the goats remains unclear. The aim of this study was to detect the expression of in goat subcutaneous and intramuscular adipocytes, temporal expression profiles of the , and overexpressed on the differentiation of different adipocytes. In this study, PCR amplification successfully cloned the goat gene with a fragment length of 521 bp. In addition, the time point of highest expression of differed between these two adipocytes differentiation processes. Overexpression of NENF in intramuscular and subcutaneous adipocytes promoted the expression levels of differentiation markers and , which in turn promoted the differentiation of intramuscular and subcutaneous adipocytes. This study will provide basic data for further study of the role of goats in goat adipocyte differentiation and for the final elucidation of its molecular mechanisms in regulating goat adipocyte deposition.