AI Article Synopsis
- - Follicular Helper T (TFH) cells are a CD4 T cell type that helps B cells produce high-affinity antibodies, which are essential for long-lasting immunity against viruses.
- - The study focuses on how virus-specific TFH cells commit to their fate during the early phase of acute viral infections, using a mouse model of LCMV to analyze these processes.
- - Researchers employed methods like flow cytometry and retroviral transduction to investigate and manipulate the early differentiation of TFH cells, aiding in the understanding of immune responses and vaccine development.
Article Abstract
Follicular Helper T (TFH) cells are perceived as an independent CD4 T cell lineage that assists cognate B cells in producing high-affinity antibodies, thus establishing long-term humoral immunity. During acute viral infection, the fate commitment of virus-specific TFH cells is determined in the early infection phase, and investigations of the early-differentiated TFH cells are crucial in understanding T cell-dependent humoral immunity and optimizing vaccine design. In the study, using a mouse model of acute lymphocytic choriomeningitis virus (LCMV) infection and the TCR-transgenic SMARTA (SM) mouse with CD4 T cells specifically recognizing LCMV glycoprotein epitope I-AGP66-77, we described procedures to access the early fate commitment of virus-specific TFH cells based on flow cytometry stainings. Furthermore, by exploiting retroviral transduction of SM CD4 T cells, methods to manipulate gene expression in early-differentiated virus-specific TFH cells are also provided. Hence, these methods will help in studies exploring the mechanism(s) underlying the early commitment of virus-specific TFH cells.
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| http://dx.doi.org/10.3791/66752 | DOI Listing |
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