AI Article Synopsis
- A new portable photothermal aptasensor for detecting ochratoxin A (OTA) was developed, which uses a thermometer and avoids the need for expensive equipment.
- The sensor works by utilizing a clever mechanism where binding of aptamers to OTA triggers a series of reactions that allow the concentration of OTA to be quantified.
- It has a detection limit of 2.28 nM in a clean buffer and 2.43 nM in grape juice, making it suitable for testing in low-resource environments.
Article Abstract
Aptasensors for detection of ochratoxin A (OTA) have been extensively studied, but the majority of them require costly and large-scale equipment as signal readers. Herein, a photothermal aptasensor capable of portable detection of OTA through a thermometer was developed on basis of aptamer structural switching and rolling circle amplification (RCA)-enriched DNAzyme. Oligonucleotides and alkaline phosphatase (ALP) modified magnetic beads were prepared. The binding of aptamers to OTA led to the release of ALP labeled complementary DNA. After magnetic separation, ALP catalyzed the padlock dephosphorylation, inhibiting the subsequent RCA reaction. This process converted the OTA concentration into the amount of the photothermal reagent oxTMB produced from the catalytic reaction induced by RCA-enriched DNAzyme. Under the optimal conditions, the detection limit (LOD) of this aptasensor was 2.28 nM in a clean buffer, while the LOD reached 2.43 nM in 2 % grape juice. The good performance of the photothermal aptasensor makes it possible to measure OTA pollution in low resource environments.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ijbiomac.2024.132279 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Portable self-powered electrochemical aptasensor for ultrasensitive and real-time detection of microcystin-RR based on hydrovoltaic-photothermal coupling effect.
Biosens Bioelectron
January 2025
Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, School of Petrochemical Engineering, Changzhou University, Changzhou, Jiangsu, 213164, PR China. Electronic address:
Coupling different energy harvesting technologies to obtain an excellent output signal is essential for the development of high-performance self-powered electrochemical sensors. Herein, a novel hydrovoltaic-photothermal coupling self-powered electrochemical aptasensing platform was designed for sensitive detection of microcystin (MC-RR) with a digital multimeter as a direct visual readout strategy. The straightforward ultrasonic method was employed to synthesize polyaniline (PANI) and bismuth oxybromide (BiOBr) nanosheets, which were then integrated as active components in a hydrovoltaic device.
View Article and Find Full Text PDFA novel multimodal aptasensor for Patulin detection in fruit products based on high-performance RuMOF@hydrogel and versatile pericarp-derived carbonized polymer dots.
Food Chem
December 2024
Key Laboratory of the Environmental Medicine and Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China. Electronic address:
Patulin (PAT) is a widespread fruit toxin. Trace-level PAT exposure can cause serious harm to human health. Herein, a multimodal PAT aptasensor was designed based on Ru(bpy)-based metal organic framework composited hydrogel (RuMOF@hydrogel) and versatile banana peel-derived carbonized polymer dots (BPPDs).
View Article and Find Full Text PDFSandwich Layer-Modified Ω-Shaped Fiber-Optic LSPR Enables the Development of an Aptasensor for a Cytosensing-Photothermal Therapy Circuit.
ACS Sens
September 2024
Research Center of Analytical Instrumentation, School of Mechanical Engineering, Sichuan University, Chengdu 610065, China.
The metastasis of cancer cells is a principal cause of morbidity and mortality in cancer. The combination of a cytosensor and photothermal therapy (PTT) cannot completely eliminate cancer cells at one time. Hence, this study aimed to design a localized surface plasmonic resonance (LSPR)-based aptasensor for a circuit of cytosensing-PTT (COCP).
View Article and Find Full Text PDFA photothermal aptasensor based on rolling circle amplification-enriched DNAzyme for portable detection of ochratoxin A in grape juice.
Int J Biol Macromol
June 2024
Key Laboratory of Quality and Safety Control of Citrus Fruits, Ministry of Agriculture and Rural Affairs, Southwest University, Chongqing 400712, PR China; Laboratory of Quality & Safety Risk Assessment for Citrus Products (Chongqing), Ministry of Agriculture and Rural Affairs, Citrus Research Institute, Southwest University, Chongqing 400712, PR China; National Citrus Engineering Research Center, Chongqing 400712, PR China. Electronic address:
Article Synopsis
- A new portable photothermal aptasensor for detecting ochratoxin A (OTA) was developed, which uses a thermometer and avoids the need for expensive equipment.
- The sensor works by utilizing a clever mechanism where binding of aptamers to OTA triggers a series of reactions that allow the concentration of OTA to be quantified.
- It has a detection limit of 2.28 nM in a clean buffer and 2.43 nM in grape juice, making it suitable for testing in low-resource environments.
Magnetically Controlled Photothermal, Colorimetric, and Fluorescence Trimode Assay for Gastric Cancer Exosomes Based on Acid-Induced Decomposition of CP/Mn-PBA DSNBs.
Anal Chem
March 2024
Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, Xuzhou 221004, China.
Article Synopsis
- Researchers developed a new biosensor that can accurately detect exosomes from gastric cancer cells, which could help in noninvasive cancer diagnosis through liquid biopsies.
- The sensor uses advanced materials, specifically nanocomposites, that exhibit photothermal and colorimetric properties, allowing for multiple detection methods based on exosome concentration.
- Testing the sensor with human serum samples showed its effectiveness in distinguishing between gastric cancer patients and healthy individuals.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!