Evaluation of phyto-gallic acid as a potential inhibitor of efflux pump mediated tetracycline resistance: an and in-silico study.

Plants contain many bioactive compounds with potent antibacterial and efflux pump inhibitory activity (EPI). In this study, gallic acid extracted from pomegranate molasses by analytical HPLC holds promise as an EPI drug for mediated tetracycline resistance, it lowered the bacterial resistance and reversed the mechanism family efflux pump, using molecular technique and molecular docking analysis. Extracted gallic acid combined with tetracycline demonstrated a significant decrease in the minimal inhibitory concentration MIC compared to its single activity. Similarly, little growth and lower fluorescence of were observed on ethidium bromide (2.5 mg/mL) agar plates, indicating a reversible efflux pump mechanism and a potent EPI activity. Molecular docking demonstrated a promising affinity binding energy between gallic acid and efflux genes, opening a new baseline in bacterial infection treatment. PCR for K and A/B genes failed to show any relation between genes and gallic acid.