Black carrot anthocyanins have gained increasing attention as natural coloring agent, owing to their higher stability than anthocyanins from berries. The stability has been attributed to their higher degree of acylation. This study investigated the impact of acylation on the stability of individual anthocyanins during storage in light and darkness. We hypothesized that the acylated anthocyanins would be more stable than the non-acylated ones. The major five anthocyanins were fractioned by semi-preparative HPLC and stored at pH 4.5 in light and darkness to investigate how acylation affected the stability. The stability was evaluated by absorption spectroscopy and mass spectrometry (MS). Two of the anthocyanins were non-acylated; 3-xylosyl(glucosyl)galactoside and cyanidin 3-xylosylgalactoside, and three were acylated; cyanidin 3-xylosyl(sinapolyglucosyl)galacto-side, cyanidin 3-xylosyl(feruloylglu-cosyl)galactoside, and cyanidin 3-xylosyl(coumaroyl-glucosyl)galactoside. Both methods (spectroscopy and MS) showed a clear effect of acylation when stored in light, but surprisingly the two non-acylated anthocyanins, showed higher stability than the three acylated ones.
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http://dx.doi.org/10.1016/j.foodres.2024.114382 | DOI Listing |
AoB Plants
January 2025
Department of Biodiversity and Biostatistics, Institute of Biosciences of Botucatu, São Paulo State University (UNESP), Professor Antônio Celso Wagner Zagnin street, 250, District of Rubião Júnior, 18618-970, Botucatu City, São Paulo State, Brazil.
The anatomical and cytological characteristics of the mucilage-secretory system have been widely studied in Malvaceae. However, conflicting information regarding the morphological nature of secretory structures exists, and some remain poorly understood. In this sense, some secretory structures in Malvaceae are not characterized as typical isolated idioblasts, canals, or cavities.
View Article and Find Full Text PDFFilm-coupled plasmonic resonators offer efficient platforms for light enhancement due to the excitation of gap surface plasmons (GSPs) at metal-insulator-metal interfaces, where electromagnetic energy is stored within the spacer. In applications like biosensing and spontaneous emission control, spatial overlap between the target molecule and plasmonic hotspots is essential. Here, we propose utilizing the controllable, efficient light enhancement capabilities of a specifically designed GSP disk resonator for biosensing and spontaneous emission enhancement.
View Article and Find Full Text PDFTalanta
January 2025
Institute of Environmental Science and School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, 030006, China. Electronic address:
Plant Dis
January 2025
Henan Normal University, College of Life Sciences, Xinxiang, Xinxiang, Henan, China, 453007.
Echinacea purpurea (Eastern Purple Coneflower) is a perennial herbaceous plant belonging to the Asteraceae. It originated from North America and is cultivated all over the world. Extracts of E.
View Article and Find Full Text PDFPeerJ
January 2025
Department of Biology, School of Sciences and Humanities, Nazarbayev University, Astana, Kazakhstan.
Background: Chitosan nanoparticles (CsNPs) are an effective and inexpensive approach for DNA delivery into live cells. However, most CsNP synthesis protocols are not optimized to allow long-term storage of CsNPs without loss of function. Here, we describe a protocol for CsNP synthesis, lyophilization, and sonication, to store CsNPs and maintain transfection efficiency.
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