sp. nov., a novel denitrifying bacteria isolated from the Yellow Sea and transfer of and to the genus as comb. nov. and nom. nov.

A Gram-stain-negative and rod-shaped bacterium, designated strain CY04, was isolated from a sediment sample collected from the Yellow Sea. CY04 exhibited the highest 16S rRNA gene sequence similarity of 98.7 % to CY05, followed by the similarities of 98.6 %, 98.0 and 98.0 % to DSW4-44, W43 and QS115 respectively. Phylogenetic analysis based on 16S rRNA gene and phylogenomic analysis based on genome sequences revealed that CY04 formed a robust cluster with CY05, DSW4-44, W43 and QS115. Calculated digital DNA-DNA hybridisation and average nucleotide identity values between CY04 and its closely related species were 22.2-23.7 % and 79.0-81.2 % respectively. Cells of CY04 were strictly aerobic, non-motile and positive for catalase, oxidase and denitrification. CY04 harboured a set of genes encoding the enzymes involved in denitrification. Growth occurred at 10-30 °C (optimum, 20 °C), at pH 6.5-9.5 (optimum, pH 8.0) and with 1-6 % (w/v) (optimum, 2.5 %,) NaCl. The major component of the fatty acids was summed feature 8 (Cω7 and/or Cω6). The isoprenoid quinone was Q-10. Results of the phenotypic, chemotaxonomic and molecular study indicate that strain CY04 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CY04 (=MCCC 1K08635=KCTC 62199). It is also proposed that and should be reclassified as comb. nov. and nom. nov. An emended description of the genus is also proposed.