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Department of Systems Biotechnology and Center for Antibiotic Resistome, Chung-Ang University, Anseong, South Korea.
Published: June 2024
AI Article Synopsis
Article Abstract
Unlabelled: , a common commensal bacterium found on human skin, can cause infections in clinical settings, and the presence of antibiotic resistance genes (ARGs) impedes the treatment of infections. However, studies characterizing the ARGs in with regard to genomic and ecological diversities are limited. Thus, we performed a comprehensive and comparative analysis of 405 high-quality genomes, including those of 35 environmental isolates from the Han River, to investigate the genomic diversity of antibiotic resistance in this pathogen. Comparative genomic analysis revealed the prevalence of ARGs in genomes associated with multi-locus sequence types. The genes encoding dihydrofolate reductase () and multidrug efflux pump () were genome-wide core ARGs. β-Lactam class ARGs were also highly prevalent in the genomes, which was consistent with the resistance phenotype observed in river isolates. Furthermore, we identified chloramphenicol acetyltransferase genes () in the plasmid-like sequences of the six river isolates, which have not been reported previously in genomes. These genes were identical to those harbored by the plasmids and associated with the insertion sequence 6 family transposases, homologous to those found in s plasmids, suggesting the possibility of horizontal gene transfer between these Gram-positive pathogens. Comparison of the ARG and virulence factor profiles between and s genomes revealed that these two species were clearly distinguished, suggesting genomic demarcation despite ecological overlap. Our findings provide a comprehensive understanding of the genomic diversity of antibiotic resistance in .
Importance: A comprehensive understanding of the antibiotic resistance gene (ARG) profiles of the skin commensal bacterium and opportunistic pathogen needs to be documented from a genomic point of view. Our study encompasses a comparative analysis of entire genomes from various habitats, including those of 35 environmental isolates from the Han River sequenced in this study. Our results shed light on the distribution and diversity of ARGs within different multi-locus sequence types, providing valuable insights into the ecological and genetic factors associated with antibiotic resistance. A comparison between and revealed marked differences in ARG and virulence factor profiles, despite their overlapping ecological niches.
