Background: Our aim was to establish which of the current and novel storage fluids caused the least harm to the endothelium of the saphenous vein.
Methods: Unmanipulated samples of saphenous vein were obtained. This vein was exposed to four fluids, DuraGraft, Plasma-Lyte, autologous blood and sodium chloride, and a control medium. The expression of endothelial selectin (E-selectin) and endothelial nitric oxide synthase (eNOS) was measured using confocal microscopy.
Results: We identified and quantified platelet endothelial cell adhesion molecules, vascular cell adhesion molecules and endothelial nitric oxide synthase 3 on the endothelium. The results were widely variable. The protein expression of endothelial selectin ( = .17) and endothelial nitric oxide synthase 3 ( = .73) showed no statistically significant differences in levels of preservation when the different solutions were compared.
Conclusions: In this study, we could not determine that any of the solutions were superior at preserving saphenous vein endothelium.
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