AI Article Synopsis
- Influenza A viruses (IAVs) are a significant public health threat, and developing effective prevention and treatment methods is critical.
- The study focused on a monoclonal antibody (FNA1) targeting the neuraminidase (NA) protein of H1N1 and H5N1, showing strong antiviral properties but not affecting H3N2 or H7N9.
- Specific residues in the NA protein were identified as essential for FNA1 binding; however, more research is needed to confirm FNA1's effectiveness as a treatment for H1N1 and H5N1 infections.
Article Abstract
Influenza A viruses (IAVs) continue to pose a huge threat to public health, and their prevention and treatment remain major international issues. Neuraminidase (NA) is the second most abundant surface glycoprotein on influenza viruses, and antibodies to NA have been shown to be effective against influenza infection. In this study, we generated a monoclonal antibody (mAb), named FNA1, directed toward N1 NAs. FNA1 reacted with H1N1 and H5N1 NA, but failed to react with the NA proteins of H3N2 and H7N9. In vitro, FNA1 displayed potent antiviral activity that mediated both NA inhibition (NI) and blocking of pseudovirus release. Moreover, residues 219, 254, 358, and 388 in the NA protein were critical for FNA1 binding to H1N1 NA. However, further validation is necessary to confirm whether FNA1 mAb is indeed a good inhibitor against NA for application against H1N1 and H5N1 viruses.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11081314 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0302865 | PLOS |
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