is an important opportunistic human pathogen well known to develop resistance to antifungal drugs. Due to their numerous desirable qualities, antimicrobial lipopeptides have gained significant attention as promising candidates for antifungal drugs. In the present study, two bioactive lipopeptides (AF and AF m/z 1071.5 and 1085.5, respectively), coproduced and purified from RLID12.1, consist of seven amino acid residues with lipid moieties In our previous studies, the reversed phased-HPLC purified lipopeptides demonstrated broad-spectrum of antifungal activities against over 110 non and mycelial fungi. Two lipopeptides triggered membrane permeabilization of cells, as confirmed by propidium iodide-based flow cytometry, with PI uptake up to 99% demonstrating fungicidal effects. Metabolic inactivation in treated cells was confirmed by FUN-1-based confocal microscopy. Together, the results indicate that these lipopeptides have potentials to be developed into a new set of antifungals for combating fungal infections.
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| http://dx.doi.org/10.3389/abp.2024.11999 | DOI Listing |
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