AI Article Synopsis
- Antimicrobial resistance presents a major health risk due to the serious infections caused by drug-resistant microbes, highlighting the need for effective identification methods.
- This study introduces a sandwich-type electrochemical DNA biosensor using polyadenine-DNA tetrahedron probes, optimizing conditions for improved detection capabilities.
- The biosensor demonstrated strong performance with a detection limit of 1 fM and successfully identified multiple drug resistance genes, showcasing its practical application for rapid detection of antibiotic-resistant strains.
Article Abstract
Antimicrobial resistance poses a serious threat to human health due to the high morbidity and mortality caused by drug-resistant microbial infections. Therefore, the development of rapid, sensitive and selective identification methods is key to improving the survival rate of patients. In this paper, a sandwich-type electrochemical DNA biosensor based on a polyadenine-DNA tetrahedron probe was constructed. The key experimental conditions were optimized, including the length of polyadenine, the concentration of the polyadenine DNA tetrahedron, the concentration of the signal probe and the hybridization time. At the same time, poly-avidin-HRP80 was used to enhance the electrochemical detection signal. Finally, excellent biosensor performance was achieved, and the detection limit for the synthetic DNA target was as low as 1 fM. In addition, we verified the practicability of the system by analyzing with the MCR-1 plasmid and realized multi-channel detection of the drug resistance genes MCR-1, blaNDM, blaKPC and blaOXA. With the ideal electrochemical interface, the polyA-based biosensor exhibits excellent stability, which provides powerful technical support for the rapid detection of antibiotic-resistant strains in the field.
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| http://dx.doi.org/10.1039/d4an00488d | DOI Listing |
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