Hybrid genome-mining/N-NMR was used to target compounds containing piperazate (Piz) residues, leading to the discovery of caveamides A () and B () from sp. strain BE230, isolated from New Rankin Cave (Missouri). Caveamides are highly dynamic molecules containing an unprecedented β-ketoamide polyketide fragment, two Piz residues, and a new -methyl-cyclohexenylalanine residue. Caveamide B () exhibited nanomolar cytotoxicity against several cancer cell lines and nanomolar antimicrobial activity against MRSA and .
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