AI Article Synopsis
- - Messenger RNA vaccines typically don't target dendritic cells (DCs), which are crucial for presenting antigens effectively; this study focuses on a new approach to enhance DC targeting using engineered virus-like particles.
- - These engineered particles are designed to deliver mRNA that instructs cells to produce the Spike protein of SARS-CoV-2 or herpes simplex virus proteins, allowing for more effective immune responses.
- - When tested in mice, the DC-targeting mRNA vaccine produced significantly better immune responses compared to standard vaccines, providing stronger protection against both SARS-CoV-2 and herpes simplex virus 1.
Article Abstract
Messenger RNA vaccines lack specificity for dendritic cells (DCs)-the most effective cells at antigen presentation. Here we report the design and performance of a DC-targeting virus-like particle pseudotyped with an engineered Sindbis-virus glycoprotein that recognizes a surface protein on DCs, and packaging mRNA encoding for the Spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) or for the glycoproteins B and D of herpes simplex virus 1. Injection of the DC-targeting SARS-CoV-2 mRNA vaccine in the footpad of mice led to substantially higher and durable antigen-specific immunoglobulin-G titres and cellular immune responses than untargeted virus-like particles and lipid-nanoparticle formulations. The vaccines also protected the mice from infection with SARS-CoV-2 or with herpes simplex virus 1. Virus-like particles with preferential uptake by DCs may facilitate the development of potent prophylactic and therapeutic vaccines.
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| http://dx.doi.org/10.1038/s41551-024-01208-4 | DOI Listing |
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