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Development of a cloud-based flow rate tool for eNAMPT biomarker detection. | LitMetric

Development of a cloud-based flow rate tool for eNAMPT biomarker detection.

PNAS Nexus

Department of Biomedical Engineering, The University of Arizona, 1127 E. James E. Rogers Way, Tucson, AZ 85721, USA.

Published: May 2024

AI Article Synopsis

  • Increased levels of extracellular nicotinamide phosphoribosyltransferase (eNAMPT) are identified as a valuable biomarker for inflammatory diseases, and a monoclonal antibody targeting eNAMPT has been shown to reduce inflammation in animal studies.
  • A rapid point-of-care (POC) test was developed using a particle immunoagglutination assay on a paper microfluidic platform, allowing for eNAMPT detection in plasma within a minute and analyzed via smartphone technology.
  • The assay demonstrated impressive sensitivity with a limit of detection between 1-20 pg/mL and effectively differentiated between varying levels of eNAMPT in human samples, indicating its potential for improving patient stratification and therapeutic decision-making in clinical settings.*

Article Abstract

Increased levels of extracellular nicotinamide phosphoribosyltransferase (eNAMPT) are increasingly recognized as a highly useful biomarker of inflammatory disease and disease severity. In preclinical animal studies, a monoclonal antibody that neutralizes eNAMPT has been generated to successfully reduce the extent of inflammatory cascade activation. Thus, the rapid detection of eNAMPT concentration in plasma samples at the point of care (POC) would be of great utility in assessing the benefit of administering an anti-eNAMPT therapeutic. To determine the feasibility of this POC test, we conducted a particle immunoagglutination assay on a paper microfluidic platform and quantified its extent with a flow rate measurement in less than 1 min. A smartphone and cloud-based Google Colab were used to analyze the flow rates automatically. A horizontal flow model and an immunoagglutination binding model were evaluated to optimize the detection time, sample dilution, and particle concentration. This assay successfully detected eNAMPT in both human whole blood and plasma samples (diluted to 10 and 1%), with the limit of detection of 1-20 pg/mL (equivalent to 0.1-0.2 ng/mL in undiluted blood and plasma) and a linear range of 5-40 pg/mL. Furthermore, the smartphone POC assay distinguished clinical samples with low, mid, and high eNAMPT concentrations. Together, these results indicate this POC assay, which utilizes low-cost materials, time-effective methods, and a straightforward immunoassay (without surface immobilization), may reliably allow rapid determination of eNAMPT blood/plasma levels to advantage patient stratification in clinical trials and guide ALT-100 mAb therapeutic decision-making.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11071447PMC
http://dx.doi.org/10.1093/pnasnexus/pgae173DOI Listing

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