Purpose: To assess the intraday repeatability of macular architecture measurements in glaucomatous and non-glaucomatous patients using spectral-domain optical coherence tomography (SD-OCT) and to evaluate the independence from intraindividual intraocular pressure (IOP) fluctuations.
Methods: In this single-center, time-point comparison study, 88 eyes with glaucoma, 53 eyes with ocular hypertension (OHT), and 253 healthy eyes underwent two standardized SD-OCT and intraocular pressure (IOP) measurements on the same day with a 5-h time gap. Bland-Altman plots, intraclass correlation coefficients (ICC), and random-effects model were used to analyze repeatability of entire retinal thickness, retinal nerve fiber layer, ganglion cell layer, inner plexiform layer, and inner nuclear layer measurements.
Results: Intraday measurements were highly reproducible in all 3 groups. ICC were greater than 0.90, respectively. The pairwise comparisons of morphometric parameters showed a statistically significant difference (P < 0.001, respectively) between groups (glaucoma vs. control, glaucoma vs. OHT) and a significant influence of time points. No correlation was found between IOP fluctuations and morphometric parameters (P > 0.05, respectively), except for a weak positive correlation with GCL (rho = 0.109, P = 0.031).
Conclusions: The evaluation of macular morphometric parameters of SD-OCT showed a high intraday repeatability and an excellent degree of agreement in glaucoma, ocular hypertension, and healthy groups. The fixed effects of time points were statistically significant. Except for a weak positive correlation of ganglion cell layer, variability did not appear to be affected by intraday IOP changes. Additional research is required to fully understand the impact of IOP fluctuations on macular morphometric parameters, considering the small observed IOP changes.
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http://dx.doi.org/10.1007/s00417-024-06498-7 | DOI Listing |
SLAS Discov
December 2024
Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Science, China Jiliang University, Hangzhou, 310018, China. Electronic address:
Neuron-specific enolase (NSE) is currently the most reliable biomarker for small cell lung cancer (SCLC), which is important for disease monitoring, clinical evaluation and diagnosis. However, traditional methods suffer from various disadvantages, including instability, complexity, time-consuming operations, and the necessity for standards. In this study, we developed a calibration-free concentration analysis (CFCA) method based on surface plasmon resonance (SPR) technology, to accurately quantify the active concentration of NSE without relying on any standards.
View Article and Find Full Text PDFPLoS One
November 2024
Univ. Grenoble Alpes, Inserm U1300 -HP2, CHU Grenoble Alpes, Grenoble, France.
Introduction: Current-induced vasodilation (CIV) can be used to assess the prostacyclin (PGI2) pathway. This study, for the first time, evaluated the reproducibility of several protocols used to mediate a CIV.
Methods: Three CIV protocols were evaluated in 10 healthy participants who completed four testing sessions.
Anal Chim Acta
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Applied Metabolomics Research Group, Hospital Del Mar Research Institute, Barcelona, Spain. Electronic address:
Background: Estradiol (E2) is a female sex hormone involved in several biological processes. Although E2 levels are commonly measured in blood samples, the use of non-invasive techniques (e.g.
View Article and Find Full Text PDFFood Chem
February 2025
School of Chemistry and Chemical Engineering, Southwest University, No. 2 Tiansheng Road, Beibei, Chongqing 400715, PR China; Chinese-Hungarian Cooperative Research Centre for Food Science, Chongqing 400715, PR China. Electronic address:
J Chromatogr A
December 2024
Department of Organic and Macromolecular Chemistry, Ghent University, Ghent, 9000, Belgium. Electronic address:
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