Draft genome sequence and morphological data of PLACP1, a thermophilic chloramphenicol-resistant bacterium isolated from thermophilic sludge.

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Department of Fermentation Science, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1 Sakuragaoka 1-chome, Setagaya-ku, Tokyo 156-8502, Japan.

Published: June 2024

AI Article Synopsis

  • PLACP1 is a thermophilic, aerobic Actinomyces bacterium found in compost that aids in breaking down organic matter and may host antibiotic-resistance genes (ARGs) during composting.
  • It was isolated from thermophilic sludge under specific growth conditions, showing characteristics such as being Gram-stain-positive and having long, branched cells.
  • The genome of PLACP1, which is 3,395,140 bp long and contains 3,368 genes (including many related to ARGs), has been sequenced and deposited in a public database to support further research on antibiotic resistance and its role in organic matter degradation.

Article Abstract

is a thermophilic aerobic Actinomyces often found in compost that is suggested to play a primary role in the degradation of organic matter and is a potential antibiotic-resistance gene (ARG)-hosting bacterium during the composting process. PLACP1 was isolated from thermophilic sludge on a Columbia plate supplemented with chloramphenicol. PLACP1 was Gram-stain-positive with cells longer than 20 μm that branched and intertwined with each other. A draft genome sequence of PLACP1 was generated using the Illumina NovaSeq system and deposited in the National Center for Biotechnology Information database under the BioProject accession numbers PRJDB17484 and SAMD00736731. The genome sequence comprised 3,395,140 bp, with 57.97 % GC content and 3,368 genes, including 3,267 protein-coding, 6 rRNA, and 56 tRNA genes. Based on the Comprehensive Antibiotic Resistance Database, 237 predicted gene products were related to ARGs, including 44 macrolide antibiotic-related genes (19 %) as the largest group. This dataset will be beneficial for the morphological identification, comparative genomic analyses, and ARG research in the genus .

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11068547PMC
http://dx.doi.org/10.1016/j.dib.2024.110447DOI Listing

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