AI Article Synopsis
- The study focuses on the increasing isolation rates of carbapenem-resistant Enterobacteriaceae complexes (CREC) and aims to understand their molecular characteristics and associated risk factors.
- Researchers used advanced techniques like MALDI-TOF MS and PCR to analyze 32 isolated strains, finding that they were mostly resistant to antibiotics but sensitive to polymyxin B and tigecycline.
- The findings indicate a rising prevalence of CREC in the hospital, with evidence of genetic homology among strains and the ability to transfer resistance genes, highlighting the need for ongoing monitoring and control measures.
Article Abstract
Purpose: The isolation rate of carbapenem-resistant complex (CREC) is continuously increasing. The aims of this study were to investigate the molecular characteristics and risk factors associated with CREC infections.
Methods: Bacterial species were identified using the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) (Bruker Daltonik GmbH, Bremen, Germany), and the gene was utilized for further typing. Antimicrobial susceptibilities were assessed through the MicroScan WalkAway 96 Plus system (Siemens, Germany) and the microbroth dilution method. Antimicrobial resistance genes were screened through polymerase chain reaction (PCR), while the homologous relationship was assessed using multilocus sequence typing (MLST). Conjugation experiments were performed to verify whether the plasmid could be transferred. Additionally, logistic regression model was employed to analyze risk factors for CREC infections.
Results: 32 strains of CREC bacteria were isolated during the study, yet only 20 were retained for preservation. While the isolates demonstrated resistance to the majority of antibiotics, they exhibited high sensitivity to polymyxin B and tigecycline. All isolates carried the resistance gene, including 13 isolates and 7 isolates. MLST homology analysis revealed the presence of seven known ST types and one new ST type. Conjugation experiments confirmed that 13 isolates were capable of transferring the resistance gene to strain EC600. Single-factor analysis identified multiple primary risk factors for CREC infection, but multivariate analysis did not reveal independent risk factors.
Conclusion: This study investigates the molecular characteristics and risk factors associated with CREC infections. The detection rate of CREC strains in our hospital is continuously rising and homology analysis suggested that strains might spread in our hospital, emphasizing the importance of implementing effective preventive measures to control the horizontal transmission of plasmid-mediated antimicrobial resistance genes.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11067929 | PMC |
| http://dx.doi.org/10.2147/IDR.S447857 | DOI Listing |
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