Spectral flow cytometry: Fundamentals and future impact.

Methods Cell Biol

Bindley Bioscience Center, Purdue University, West Lafayette, IN, United States.

Published: May 2024

AI Article Synopsis

  • Spectral flow cytometry is gaining traction in the scientific community for single-cell analysis and shows prospects for significant growth.
  • The technology is being integrated into clinical labs, indicating a trend toward wider acceptance, but challenges like misunderstandings and misuse remain with new methodologies.
  • This chapter provides a beginner-friendly introduction to the scientific principles behind spectral cytometry, focusing on unmixing processes for data analysis.

Article Abstract

Spectral flow cytometry has emerged as a significant player in the cytometry marketplace, with the potential for rapid growth. Despite a slow start, the technology has made significant strides in advancing various areas of single-cell analysis utilized by the scientific community. The integration of spectral cytometry into clinical laboratories and diagnostic processes is currently underway and is expected to garner a significant level of widespread acceptance in the near future. However, incorporating a new methodological approach into existing research programs can lead to misunderstandings or even misuse. This chapter offers an introductory yet comprehensive explanation of the scientific principles that form the foundation of spectral cytometry. Specifically, it delves into the unmixing processes that are utilized for data analysis. This overview is designed for those who are new to the field and seeking an informative guide to this exciting emerging technology.

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Source
http://dx.doi.org/10.1016/bs.mcb.2024.02.022DOI Listing

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