AI Article Synopsis
- The study focuses on how two bacteria, Metasolibacillus sp. ES129 and Oceanobacillus sp. ES111, efficiently convert harmful selenite into useful elemental selenium nanoparticles (SeNPs).
- The highest transformation of selenite to SeNPs occurs at specific concentrations, with ES129 and ES111 showing different optimal levels.
- Transcriptome analysis reveals that multiple genetic pathways are involved in this process, highlighting the potential for using these bacteria in environmental cleanup efforts related to selenite contamination.
Article Abstract
The microbial reduction of selenite to elemental selenium nanoparticles (SeNPs) is thought to be an effective detoxification process of selenite for many bacteria. In this study, Metasolibacillus sp. ES129 and Oceanobacillus sp. ES111 with high selenite reduction efficiency or tolerance were selected for systematic and comparative studies on their performance in selenite removal and valuable SeNPs recovery. The kinetic monitoring of selenite reduction showed that the highest transformation efficiency of selenite to SeNPs was achieved at a concentration of 4.24 mM for ES129 and 4.88 mM for ES111. Ultramicroscopic analysis suggested that the SeNPs produced by ES111 and ES129 had been formed in cytoplasm and subsequently released to extracellular space through cell lysis process. Furthermore, the transcriptome analysis indicated that the expression of genes involved in bacillithiol biosynthesis, selenocompound metabolism and proline metabolism were significantly up-regulated during selenite reduction, suggesting that the transformation of selenite to Se may involve multiple pathways. Besides, the up-regulation of genes associated with nucleotide excision repair and antioxidation-related enzymes may enhance the tolerance of bacteria to selenite. Generally, the exploration of selenite reduction and tolerance mechanisms of the highly selenite-tolerant bacteria is of great significance for the effective utilization of microorganisms for environmental remediation.
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| http://dx.doi.org/10.1016/j.jhazmat.2024.134491 | DOI Listing |
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