AI Article Synopsis
- Copper/zinc superoxide dismutase (Cu/Zn-SOD), identified as PmECSOD in this study, plays a crucial role in eliminating reactive oxygen species (ROS) and protecting the body's oxygen balance.
- Researchers used multi-step high-performance liquid chromatography (HPLC) and Mass Spectrometry (MS) to isolate PmECSOD from the serum of Pinctada fucata martensii, revealing a cDNA length of 1864 bp encoding a 473 amino acid protein.
- The PmECSOD transcript was found to be highly expressed in the gills and showed peak expression in hemolymph 48 hours post-stimulation with bacteria, highlighting its potential importance in molluscan immune responses
Article Abstract
Copper/zinc superoxide dismutase (Cu/Zn-SOD) can effectively eliminate reactive oxygen species (ROS)，avoid damage from O to the body, and maintain O balance. In this study, multi-step high-performance liquid chromatography (HPLC), combined with Mass Spectrometry (MS), was used to isolate and identify Cu/Zn-SOD from the serum of Pinctada fucata martensii (P. f. martensii) and was designated as PmECSOD. With a length of 1864 bp and an open reading frame (ORF) of 1422 bp, the cDNA encodes a 473 amino acid protein. The PmECSOD transcript was detected in multiple tissues by quantitative real-time PCR (qRT-PCR), with its highest expression level being in the gills. Additionally, the temporal expression of PmECSOD mRNA in the hemolymph was highest at 48 h after in vivo stimulation with Escherichia coli and Micrococcus luteus. The results from this study provide a valuable base for further exploration of molluscan innate immunity and immune response.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.fsi.2024.109599 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!