AI Article Synopsis
- Researchers are creating a new version of the ACE2 receptor, called miniature ACE2 (mACE2), which can bind tightly to the spike protein of SARS-CoV-2.
- This engineered mACE2 is used in a novel detection method for the virus, utilizing magnetic nanoparticles for sensitivity and specificity.
- The study demonstrates that mACE2 could be a promising tool for not only diagnosing but also neutralizing SARS-CoV-2, paving the way for similar approaches against other viral diseases.
Article Abstract
As the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues its global spread, the exploration of novel therapeutic and diagnostic strategies is still needed. The virus enters host cells by binding the angiotensin-converting enzyme 2 (ACE2) receptor through the spike protein. Here, we develop an engineered, small, stable, and catalytically inactive version of ACE2, termed miniature ACE2 (mACE2), designed to bind the spike protein with high affinity. Employing a magnetic nanoparticle-based assay, we harnessed the strong binding affinity of mACE2 to develop a sensitive and specific platform for the detection or neutralization of SARS-CoV-2. Our findings highlight the potential of engineered mACE2 as a valuable tool in the fight against SARS-CoV-2. The success of developing such a small reagent based on a piecewise molecular design serves as a proof-of-concept approach for the rapid deployment of such agents to diagnose and fight other viral diseases.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11586090 | PMC |
| http://dx.doi.org/10.1021/acsabm.4c00222 | DOI Listing |
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