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| http://dx.doi.org/10.1016/j.jid.2024.04.004 | DOI Listing |
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Comparison of Tape-Stripping Platforms as Noninvasive Solutions for Skin Biomarker Discovery.
J Invest Dermatol
November 2024
DermTech, San Diego, California, USA. Electronic address:
Evaluation of Less Invasive Sampling Tools for the Diagnosis of Cutaneous Leishmaniasis.
Open Forum Infect Dis
April 2024
Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, Belgium.
Background: Diagnosis of cutaneous leishmaniasis (CL) usually relies on invasive samples, but it is unclear whether more patient-friendly tools are good alternatives for diverse lesions when used with polymerase chain reaction (PCR).
Methods: Patients with suspected CL were enrolled consecutively in a prospective diagnostic accuracy study. We compared dental broach, tape disc, and microbiopsy samples with PCR as index tests, using PCR with skin slit samples as reference test.
Comparison between ammonium formate and ammonium fluoride in the analysis of stratum corneum lipids by reversed phase chromatography coupled with high resolution mass spectrometry.
Sci Rep
January 2024
Laboratory of Cutaneous Physiopathology, San Gallicano Dermatological Institute - IRCCS, Via Elio Chianesi 53, 00144, Rome, Italy.
Article Synopsis
- Lipids are essential for the barrier function of the stratum corneum (SC), the outer layer of the skin, composed mainly of ceramides, fatty acids, and cholesterol in equal amounts.
- Liquid chromatography-mass spectrometry (LCMS) is a key technique for analyzing these lipids, but negative ion mode can complicate ceramide identification due to structural diversity.
- The introduction of fluoride in the LCMS process improves ceramide ionization and helps identify lipid classes more accurately, proving effective for analyzing skin samples.
Intrapatient comparison of atopic dermatitis skin transcriptome shows differences between tape-strips and biopsies.
Allergy
January 2024
Department of Dermatology, and Laboratory of Inflammatory Skin Diseases, Icahn School of Medicine at Mount Sinai, New York City, New York, USA.
Background: Our knowledge of etiopathogenesis of atopic dermatitis (AD) is largely derived from skin biopsies, which are associated with pain, scarring and infection. In contrast, tape-stripping is a minimally invasive, nonscarring technique to collect skin samples.
Methods: To construct a global AD skin transcriptomic profile comparing tape-strips to whole-skin biopsies, we performed RNA-seq on tape-strips and biopsies taken from the lesional skin of 20 moderate-to-severe AD patients and the skin of 20 controls.
Dermal Delivery of Diclofenac Sodium-In Vitro and In Vivo Studies.
Pharmaceutics
October 2022
Department of Pharmaceutics, UCL School of Pharmacy, 29-39 Brunswick Square, London WC1N 1AX, UK.
Previously, we reported the use of confocal Raman spectroscopy (CRS) as a novel non-invasive approach to determine drug disposition in the skin in vivo. Results obtained by CRS were found to correlate with data from the well-established in vitro permeation test (IVPT) model using human epidermis. However, these studies used simple vehicles comprising single solvents and binary or ternary solvent mixtures; to date, the utility of CRS for monitoring dermal absorption following application of complex marketed formulations has not been examined.
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