Background: Low-intensity pulsed ultrasound (LIPUS) can accelerate tooth movement and preserve tooth and bone integrity during orthodontic treatment. However, the mechanisms by which LIPUS affects tissue remodeling during orthodontic tooth movement (OTM) remain unclear. Periodontal ligament cells (PDLCs) are pivotal in maintaining periodontal tissue equilibrium when subjected to mechanical stimuli. One notable mechano-sensitive ion channel, Piezo1, can modulate cellular function in response to mechanical cues. This study aimed to elucidate the involvement of Piezo1 in the osteogenic response of force-treated PDLCs when stimulated by LIPUS.
Method: After establishing rat OTM models, LIPUS was used to stimulate rats locally. OTM distance and alveolar bone density were assessed using micro-computed tomography, and histological analyses included hematoxylin and eosin staining, tartrate-resistant acid phosphatase staining and immunohistochemical staining. GsMTx4 and Yoda1 were respectively utilized for Piezo1 functional inhibition and activation experiments in rats. We isolated human PDLCs (hPDLCs) and evaluated the effects of LIPUS on the osteogenic differentiation of force-treated hPDLCs using real-time quantitative PCR, Western blot, alkaline phosphatase and alizarin red staining. Small interfering RNA and Yoda1 were employed to validate the role of Piezo1 in this process.
Results: LIPUS promoted osteoclast differentiation and accelerated OTM in rats. Furthermore, LIPUS alleviated alveolar bone resorption under pressure and enhanced osteogenesis of force-treated PDLCs both and by downregulating Piezo1 expression. Subsequent administration of GsMTx4 in rats and siPIEZO1 transfection in hPDLCs attenuated the inhibitory effect on osteogenic differentiation under pressure, whereas LIPUS efficacy was partially mitigated. Yoda1 treatment inhibited osteogenic differentiation of hPDLCs, resulting in reduced expression of Collagen Ⅰα1 and osteocalcin in the periodontal ligament. However, LIPUS administration was able to counteract these effects.
Conclusion: This research unveils that LIPUS promotes the osteogenesis of force-treated PDLCs via downregulating Piezo1.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11061374 | PMC |
| http://dx.doi.org/10.3389/fbioe.2024.1347406 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Historical Pathways Highlighting Misgivings and Misinterpretations in the Processes of Tooth Absorption & Resorption.
J Hist Dent
January 2025
Professor Emeritus Texas A&M University, College of Dentistry, Dallas, Texas, Distinguished Adjunct Professor, Department of Cariology, Saveetha Dental College and Hospitals Saveetha Institute of Medical and Technical Sciences (SIMATS) Saveetha University, Chennai, India.
Historically the physiological or pathological loss of tooth structure in situ was deemed to be due to the 'absorption' of tooth structure due to the removal of the inorganic components of dentin and cementum by osteoclastic (dentinoclastic) cellular activity. This nomenclature and the activity that it represented was considered by almost all dental researchers and clinicians in the 1800s and early 1900s. The shift to the concept of 'resorption' occurred in the first half of the 20th century, with clarity emanating from significant research activity on the pathology of osseous structures, origin of osteoclastic cell types, and the function of periodontal ligament cells.
View Article and Find Full Text PDFSurvival of periodontal ligament myofibroblasts after short-term mechanical strain in rats and in vitro: Could myofibroblasts contribute to orthodontic relapse?
Arch Oral Biol
January 2025
Department of Dentistry-Orthodontics and Craniofacial Biology, Research Institute for Medical Innovation, Radboud university medical center, Philips van Leydenlaan 25, Nijmegen 6525 EX, the Netherlands. Electronic address:
Objectives: To investigate in vivo whether myofibroblasts formed in the PDL after exposure to short-term high experimental orthodontic forces in rats survive. To study in vitro whether human PDL fibroblasts can differentiate into myofibroblasts and survive when chemical or mechanical stimuli are removed.
Design: Nine 6-week-old male Wistar rats were used in this experiment.
Transcriptional regulation of human follicular dendritic cell-secreted protein gene by interleukin-6.
Odontology
January 2025
Department of Periodontology, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
Follicular dendritic cell-secreted protein (FDC-SP) is produced by follicular dendritic cells, periodontal ligament and junctional epithelium (JE). JE exists immediately apical to the bottom of the pocket and binds enamel with hemidesmosomes to protect the periodontium from bacterial infection. To analyze the transcriptional regulation of the FDC-SP gene by interleukin-6 (IL-6), we performed real-time PCR, Western blotting, immunofluorescence, luciferase (LUC) assays, gel mobility shift and chromatin immunoprecipitation (ChIP) assays using Ca9-22 and Sa3 gingival epithelial cells.
View Article and Find Full Text PDFAre metal-based antibacterial gels a potential alternative for disinfection in contemporary endodontics?
Evid Based Dent
January 2025
Doctoral Research Fellow and Specialty Trainee (Endodontics), School of Dental Sciences, Faculty of Medical Sciences, Newcastle University, Newcastle Upon Tyne, UK.
Aims: This study aimed to assess the effectiveness of a novel antimicrobial gel, containing copper and silver nanoparticles, for use in root canal disinfection.
Methods: Copper and silver-based gels were created in-house, using a support network of biocompatible polymers, including polyvinyl alcohol (PVA), polyvinyl pyrrolidone (PVP), and polyethylene glycol (PEG). Six experimental groups were created, three containing silver ions and three copper ions, where the PVA, PVP and PEG ratios were also adjusted in each group to test the gel's physical state.
Histological Evaluation of Sodium Iodide-Based Root Canal Filling Materials in Canine Teeth.
Materials (Basel)
December 2024
Institute of Tissue Regeneration Engineering (ITREN), Dankook University, 119 Dandae-ro, Cheonan 31116, Republic of Korea.
A novel water-soluble root canal filling material based on sodium iodide (NaI) has been developed to overcome the limitations of existing iodine-based formulations. However, the biological stability of this approach in animal studies remains unverified. This study evaluated the biocompatibility of NaI compared to commercial root canal filling materials (Calcipex II and Vitapex) in pulpectomized canine teeth to assess its clinical applicability.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!