The in vivo fate of chemotherapeutic drugs plays a vital role in understanding the therapeutic outcome, side effects, and the mechanism. However, the lack of imaging abilities of drugs, tedious labeling processes, and premature leakage of imaging agents result in loss of fidelity between the drugs and imaging signals. Herein, an amphiphilic polymer is created by copolymerization of a near-infrared-II (NIR-II) fluorophore tracer (T) and an anticancer Pt(IV) prodrug (D) of cisplatin in a hand-holding manner into one polymer chain for the first time. The obtained Polyplatin is capable of delivering the drugs and the fluorophores concomitantly at a precise D/T ratio, thereby resulting in tracking the platinum drugs and even readout of them in real-time via NIR-II imaging. Polyplatin can self-assemble into nanoparticles, referred to as Nanoplatin. Furthermore, a caspase-3 cleavable peptide that serves as an apoptosis reporter is attached to Nanoplatin, resulting in Nanoplatin that are capable of simultaneously tracking platinum drugs and evaluating the therapeutic efficacy. Overall, it is reported here the design of the first theranostic polymer with anticancer drugs, drug tracers, and drug efficacy reporters that can work in concert to provide insight into the drug fate and mechanism of action.
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http://dx.doi.org/10.1002/adma.202402452 | DOI Listing |
Pediatr Nephrol
January 2025
Cardiology Department, Faculty of Medicine (Girls), Al-Azhar University, Cairo, Egypt.
Background: Changes in cardiac function and structure as well as their association with the cardiac autonomic nervous system remain incompletely characterized in children with stage 5 chronic kidney disease (CKD) receiving hemodialysis (HD).
Methods: A prospective observational cohort study was conducted on 40 Egyptian children with CKD on regular HD compared to 40 age- and sex-matched healthy children. All participants underwent thorough clinical examination, laboratory investigations, 24-h Holter monitoring, and 2D/4D echocardiographic study (conventional and advanced modalities).
Sci Rep
January 2025
Centre for Informatics and Systems of the University of Coimbra, Department of Informatics Engineering, University of Coimbra, Coimbra, Portugal.
Assessing cognitive load using pupillography frequency features presents a persistent challenge due to the lack of consensus on optimal frequency limits. This study aims to address this challenge by exploring pupillography frequency bands and seeking clarity in defining the most effective ranges for cognitive load assessment. From a controlled experiment involving 21 programmers performing software bug inspection, our study pinpoints the optimal low-frequency (0.
View Article and Find Full Text PDFArch Gerontol Geriatr
January 2025
School of Public Health Sciences, University of Waterloo, Waterloo, Ontario, Canada. Electronic address:
Purpose: Although several studies have reported positive associations between functional social support (FSS) and memory, few have explored how other social variables, such as marital status, may affect the magnitude and direction of this association. We examined whether marital status modifies the association between FSS and memory in a sample of community-dwelling, middle-aged and older adults.
Methods: Data at three timepoints, spanning six years, were analyzed from the Tracking Cohort of the Canadian Longitudinal Study on Aging (n = 10,318).
The polymerase gamma (POLG) gene mutation is associated with mitochondria and metabolism disorders, resulting in heterogeneous responses to immunological activation and posing challenges for mitochondrial disease therapy. Optical metabolic imaging captures the autofluorescent signal of two coenzymes, NADH and FAD, and offers a label-free approach to detect cellular metabolic phenotypes, track mitochondria morphology, and quantify metabolic heterogeneity. In this study, fluorescence lifetime imaging (FLIM) of NAD(P)H and FAD revealed that POLG mutator macrophages exhibit a decreased NAD(P)H lifetime, and optical redox ratio compared to the wild-type macrophages, indicating an increased dependence on glycolysis.
View Article and Find Full Text PDFTalanta
January 2025
Department of Neurosurgery, Ningbo Medical Center Lihuili Hospital, Ningbo University, Ningbo City, Zhejiang Province, 315040, China
The considerable abundance and remarkable stability of sEVs provide substantial benefits for diagnosing Alzheimer's disease. Therefore, precise tracking subtypes of small extracellular vesicles (sEVs) is crucial for screening novel diagnostic biomarkers and developing therapeutic technologies. We propose a three-target recognition-mediated proximity ligation assay for the precise identification of sEV subtypes utilizing three specifically designed probes: one for the exosomal surface protein CD63 recognition, one for fixing the biolipid layer, and the third for the identification of distinctive protein associated with a specific subtype of sEVs (L1CAM positive sEVs).
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