AI Article Synopsis
- Biobanks containing cervical screening samples are crucial for developing tools to assess disease risk.
- Although there is interest in RNA-based biomarkers, the effectiveness of stored samples for such research has not been well documented.
- RNA extraction from 260 long-stored samples showed substantial degradation, but RT-qPCR was successful in 95% of the cases, indicating these samples are still viable for RNA-based studies after up to 14 years of storage.
Article Abstract
Biobanks of cervical screening (LBC) samples annotated with disease status are an invaluable resource to support the development of tools for the risk stratification of disease. Although there is growing interest in the assessment of RNA-based biomarkers, little is known on the suitability and durability of stored clinical samples (commonly used in cervical screening) to support RNA-based research. RNA was extracted from 260 stored LBC samples. Storage at -80°C or -25°C allowed isolation of sufficient RNA for further analysis. RNA was found to be substantially degraded according to Agilent Bioanalyser data. Despite this, RT-qPCR was successful in 95% samples tested. These data suggest that biobanked LBC samples are suitable for RNA-based assessment even if stored for up to 14 years.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2144/btn-2023-0112 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!