AI Article Synopsis
- The study investigates the roles of cleaved caspase-3 and Ki-67 index from diagnostic biopsies in predicting responses to neoadjuvant chemotherapy in breast cancer patients, focusing on different molecular subtypes and tumour characteristics.
- An analysis of 110 patients revealed significant relationships between Ki-67 levels and factors like tumour grade and molecular subtype, while caspase-3 expression correlated with treatment response according to the Sataloff classification.
- The findings suggest that while cleaved caspase-3 could indicate better responses to treatment, Ki-67 levels did not significantly differ among patients with varying treatment outcomes, highlighting the potential of these biomarkers in predicting treatment responses and guiding patient management.
Article Abstract
Introduction: Breast carcinoma is a heterogeneous disease, characterised by diverse clinical behaviour. The aim of this study was to assess how cleaved caspase-3 and Ki-67 index, evaluated on diagnostic biopsy, are related to response to neoadjuvant chemotherapy in the context of molecular subtype, post-treatment tumour, N category, and grade.
Material And Methods: A retrospective analysis was carried out among 110 breast cancer patients. Ki-67 levels and caspase-3 expression on diagnostic biopsy were explored regarding their relation to tumour grade and molecular subtype, ypT, ypN categories, and T and N categories according to Sataloff tumour response evaluation.
Results: A statistically significant relationship was found between Ki-67 levels and tumour grade K-W = 24.2932, < 0.0001; molecular subtype K-W = 28.5439, < 0.00000967538; size and invasion of the primary tumour after neoadjuvant chemotherapy K-W = 11.7944, < 0.0377169; caspase-3 expression after neoadjuvant therapy, evaluated according to the Sataloff classification χ = 5.97, df = 1, = 0.0145.
Discussion: No significant difference was found between Ki-67 expression in patients with pathological complete response, compared to those with partial and no response, a statistically significant difference in cases with different molecular subtype, histology grade, and tumour stage after neoadjuvant therapy. Cleaved caspase-3-positive breast cancer cases are often better responders to neoadjuvant therapy, but with no significant correlation to molecular subtype, high-grade categories, or tumour stage.
Conclusions: The caspase-3 and Ki-67 index on diagnostic biopsy are related to post-neoadjuvant treatment prognostic factors (ypT stage, grade), proving them useful for prediction of treatment response to neoadjuvant therapy and further patient management.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11056723 | PMC |
| http://dx.doi.org/10.5114/pm.2024.136962 | DOI Listing |
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