[This corrects the article DOI: 10.1039/D0RA05640E.].
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http://dx.doi.org/10.1039/d4ra90040e | DOI Listing |
Can Vet J
January 2025
Central Victoria Veterinary Hospital, VCA Canada, 760 Roderick Street, Victoria, British Columbia V8X 2R3 (Xie, Seguin, Brownlee, Boller); Department of Veterinary Clinical and Diagnostic Sciences, Faculty of Veterinary Medicine, University of Calgary, 3280 Hospital Drive NW, Calgary, Alberta T2N 4Z6 (Boller).
A 9-year-old neutered male cairn terrier dog was initially presented because of inappetence, increased respiratory effort, and occasional coughing. A cavitary lung mass was diagnosed using CT and removed with lung lobectomy. Histopathology of the mass revealed necrosuppurative inflammation with acid-fast rod bacteria in macrophages, with spp.
View Article and Find Full Text PDFmSystems
January 2025
Department of Infectious Diseases, School of Translational Medicine, Monash University, Melbourne, Victoria, Australia.
Unlabelled: Sequencing DNA directly from patient samples enables faster pathogen characterization compared to traditional culture-based approaches, but often yields insufficient sequence data for effective downstream analysis. CRISPR-Cas9 enrichment is designed to improve the yield of low abundance sequences but has not been thoroughly explored with Oxford Nanopore Technologies (ONT) for use in clinical bacterial epidemiology. We designed CRISPR-Cas9 guide RNAs to enrich the human pathogen , by targeting multi-locus sequence type (MLST) and transfer RNA (tRNA) genes, as well as common antimicrobial resistance (AMR) genes and the resistance-associated integron gene .
View Article and Find Full Text PDFFoodborne Pathog Dis
January 2025
Department of Cardiology, The First Affiliated Hospital of Bengbu Medical University, Bengbu, China.
Listeriosis is a rare, life-threatening bacterial infection. Prompt, appropriate antibiotic treatment is essential to save lives. We aimed to investigate antibiotic selection and listeriosis knowledge among health care providers.
View Article and Find Full Text PDFMicroorganisms
November 2024
Department of Microbiology & Virology, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy.
is a Non-Tuberculous Mycobacterium (NTM) belonging to the group, often associated with veterinary diseases, such as bovine farcy. However, it can also cause human infections and appears to be involved in Catheter-Associated Infections in immunocompromised patients. Here, we report the first Italian isolation of a strain of from a 16-year-old oncological female patient being treated at Fondazione IRCCS Policlinico San Matteo Pavia (Italy).
View Article and Find Full Text PDFAntibiotics (Basel)
December 2024
Department of Research and Development, Pathnostics, Irvine, CA 92618, USA.
: While new methods for measuring antimicrobial susceptibility have been associated with improved patient outcomes, they should also be validated using standard protocols for error rates and other test metrics. The objective of this study was to validate a novel susceptibility assay for complicated and recurrent urinary tract infections (UTIs): pooled antibiotic susceptibility testing (P-AST). This assay was compared to broth microdilution (BMD) and disk diffusion (DD), following Clinical and Laboratory Standards Institute (CLSI) guidelines for assessment of error rates and agreement.
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