sp. nov., isolated from rhizospheric soil of eggplant and genome mining for the prediction of biosynthetic gene clusters.

A Gram-stain negative, aerobic, rod-shaped, motile and flagellated novel bacterial strain, designated MAHUQ-54, was isolated from the rhizospheric soil of eggplant. The colonies were observed to be light pink coloured, smooth, spherical and 0.2-0.6 mm in diameter when grown on R2A agar medium for 2 days. MAHUQ-54 was able to grow at 15-40 °C, at pH 5.5-9.0 and in the presence of 0-0.5 % NaCl (w/v). The strain gave positive results for both catalase and oxidase tests. The strain was positive for hydrolysis of l-tyrosine, urea, Tween 20 and Tween 80. On the basis of the results of 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus and is closely related to L10 (98.8 % sequence similarity) and Feox-1 (98.2 %). MAHUQ-54 has a draft genome size of 5 994 516 bp (60 contigs), annotated with 5348 protein-coding genes, 45 tRNA and 5 rRNA genes. The average nucleotide identity (ANI) and digital DNA-DNA hybridisation (dDDH) values between MAHUQ-54 and its closest phylogenetic neighbours were 75.8-83.3 and 20.8-25.3 %, respectively. genome mining revealed that MAHUQ-54 has a significant potential for the production of novel natural products in the future. The genomic DNA G+C content was determined to be 70.4 %. The predominant isoprenoid quinone was ubiquinone-8. The major fatty acids were identified as C, summed feature 3 (comprising Cω7 and/or Cω6) and summed feature 8 (comprising Cω7 and/or Cω6). On the basis of dDDH, ANI value, genotypic analysis, chemotaxonomic and physiological data, strain MAHUQ-54 represents a novel species within the genus , for which the name sp. nov. is proposed, with MAHUQ-54 (=KACC 22001 = CGMCC 1.18515) as the type strain.