This rapid, equipment-free DNA isolation procedure using chromatography paper is a simple method that can be performed in less than 30 min and requires no wet lab experience. With minimal expense, it offers an affordable alternative for anyone wanting to explore biodiversity. It also provides an excellent option for use in classrooms or other activities that are time limited. The method works best for plants or lichens, producing stable DNA on Whatman® chromatography paper at room temperature, which can be eluted as needed.

Download full-text PDF

Source
http://dx.doi.org/10.1007/978-1-0716-3581-0_31DOI Listing

Publication Analysis

Top Keywords

rapid equipment-free
8
equipment-free dna
8
dna isolation
8
chromatography paper
8
dna
4
isolation method
4
method dna
4
dna barcoding
4
barcoding rapid
4
isolation procedure
4

Similar Publications

Pen direct writing of multiplex-LFIA for detection of thiamphenicol and tylosin in milk.

Mikrochim Acta

January 2025

Tyndall National Institute, University College Cork, Lee Maltings Complex, Dyke Parade, Cork, T12R5CP, Ireland.

Therapeutic and misuse of veterinary drugs, such as antibiotics, can increase the potential risk of residue contamination in animal-derived food products. For milk, these residual antibiotics can have an impact on efficiency in dairy processing factories, as well as economic loss, and can also cause side effects on consumer health. Lateral flow immunoassays (LFIAs) are gaining popularity for their ease of use, low cost and their fulfilment to the REASSURED (real-time connection/monitoring, easy sampling, affordable, specific, user-friendly, rapid/robust, equipment free, deliverable to end user) criteria.

View Article and Find Full Text PDF

Introduction: Hepatitis C virus (HCV) infection is a silent epidemic that needs a comprehensive and contextualised approach to manage. Access to readily available, affordable and acceptable HCV point-of-care (POC) in vitro diagnostics (IVDs) is equally required to meet the global HCV goals. However, most guidelines for evaluating these IVDs such as the WHO prequalification process and country-specific standards disproportionately focus on diagnostic performance.

View Article and Find Full Text PDF

Point-of-care test of blood Plasmodium RNA within a Pasteur pipette using a novel isothermal amplification without nucleic acid purification.

Infect Dis Poverty

October 2024

Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, No. 5, Dongdansantiao, Dongcheng District, Beijing, 10005, China.

Background: Resource-limited regions face a greater burden of infectious diseases due to limited access to molecular tests, complicating timely diagnosis and management. Current molecular point-of-care tests (POCTs) either come with high costs or lack adequate sensitivity and specificity. To facilitate better prevention and control of infectious diseases in underserved areas, we seek to address the need for molecular POCTs that better align with the World Health Organization (WHO)'s ASSURED criteria-Affordable, Sensitive, Specific, User-friendly, Rapid and robust, Equipment-free, and Deliverable to end users.

View Article and Find Full Text PDF

Inertial focusing-based Lab-on-Chip systems represent a promising technology for cell sorting in various applications, thanks to their alignment with the ASSURED criteria recommended by the World Health Organization: Affordable, Sensitive, Specific, User-friendly, Rapid and Robust, Equipment-free, and Delivered. Inertial focusing techniques using spiral microchannels offer a rapid, portable, and easy-to-prototype solution for cell sorting. Various microfluidic devices have been investigated in the literature to understand how hydrodynamic forces influence particle focusing in spiral microchannels.

View Article and Find Full Text PDF

Rapid and equipment-free identification of papaya mealybug Paracoccus marginatus based on RPA-CRISPR/Cas12a.

Pest Manag Sci

January 2025

Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, China.

Article Synopsis
  • Paracoccus marginatus, a harmful invasive pest, causes serious agricultural losses, necessitating the development of efficient detection methods for early intervention.
  • A new rapid detection system combining recombinase polymerase amplification (RPA) and CRISPR/Cas12a has been created, allowing for identification of P. marginatus from other mealybugs in about an hour, with results visible using simple tools.
  • This innovative approach utilizes common items like portable thermos cups and mini-UV torches, making pest detection accessible and effective in field settings, offering a significant advancement in pest management strategies.
View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!