AI Article Synopsis

  • The study aimed to investigate how UV-A radiation affects the aging characteristics (senescent phenotypes) of human corneal endothelial cells (hCEnCs).
  • Researchers analyzed various factors like cell shape, growth rates, and specific aging markers in hCEnCs treated with UV-A, comparing results to those from cells subjected to ionizing radiation (IR).
  • Findings showed significant overlap in gene and protein changes between UV-A and IR-induced senescent hCEnCs, indicating UV-A triggers similar aging responses while also influencing unique pathways.

Article Abstract

Purpose: The objective of this study was to investigate the senescent phenotypes of human corneal endothelial cells (hCEnCs) upon treatment with ultraviolet (UV)-A.

Methods: We assessed cell morphology, senescence-associated β-galactosidase (SA-β-gal) activity, cell proliferation and expression of senescence markers ( and ) in hCEnCs exposed to UV-A radiation, and senescent hCEnCs induced by ionizing radiation (IR) were used as positive controls. We performed RNA sequencing and proteomics analyses to compare gene and protein expression profiles between UV-A- and IR-induced senescent hCEnCs, and we also compared the results to non-senescent hCEnCs.

Results: Cells exposed to 5 J/cm2 of UV-A or to IR exhibited typical senescent phenotypes, including enlargement, increased SA-β-gal activity, decreased cell proliferation and elevated expression of and . RNA-Seq analysis revealed that 83.9% of the genes significantly upregulated and 82.6% of the genes significantly downregulated in UV-A-induced senescent hCEnCs overlapped with the genes regulated in IR-induced senescent hCEnCs. Proteomics also revealed that 93.8% of the proteins significantly upregulated in UV-A-induced senescent hCEnCs overlapped with those induced by IR. In proteomics analyses, senescent hCEnCs induced by UV-A exhibited elevated expression levels of several factors part of the senescence-associated secretory phenotype.

Conclusions: In this study, where senescence was induced by UV-A, a more physiological stress for hCEnCs compared to IR, we determined that UV-A modulated the expression of many genes and proteins typically altered upon IR treatment, a more conventional method of senescence induction, even though UV-A also modulated specific pathways unrelated to IR.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11087119PMC
http://dx.doi.org/10.18632/aging.205761DOI Listing

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