AI Article Synopsis
- GATA6 plays a crucial role in early embryonic development, especially in endoderm and mesoderm tissues.
- Researchers created a human induced pluripotent stem cell line (KSCBi017-A-1) that expresses EGFP to visualize GATA6 activity using CRISPR/Cas9 for precise gene editing.
- The new cell line is capable of differentiating into all three germ layers and can be utilized to study GATA6 expression during cell differentiation; it's available for research from the Korea National Stem Cell Bank.
Article Abstract
GATA6 is expressed during early embryogenesis and localizes to endoderm- and mesoderm-derived tissues during later embryogenesis. Here, we established a human induced pluripotent stem cell (hiPSC) line expressing EGFP under GATA6 gene. EGFP coding sequence was introduced into the C-terminus of GATA6 in KSCBi017-A hiPSCs through homologous recombination using CRISPR/Cas9 system. The successfully edited line, KSCBi017-A-1, was selected and confirmed by sequencing. The line had a normal karyotype and exhibited potential to differentiate into three germ layers while it expressed EGFP upon endoderm induction. KSCBi017-A-1 cells can be used to monitor the expression of GATA6 during differentiation. This cell line is available from Korea National Stem Cell Bank.
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| http://dx.doi.org/10.1016/j.scr.2024.103426 | DOI Listing |
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