AI Article Synopsis
- The endoplasmic reticulum (ER) and plasma membrane (PM) form contact sites (EPCSs) for exchanging materials and information, but their role in ER stress in plants is unclear.
- A study created a triple mutant in Arabidopsis thaliana lacking key VAP27 proteins, leading to disrupted ER-PM connectivity and increased unfolded protein response (UPR) signaling.
- The research highlights VAP27-1's role in EPCS remodeling under ER stress and identifies that calcium homeostasis significantly influences UPR signaling, shedding light on plant stress resistance mechanisms.
Article Abstract
The endoplasmic reticulum (ER) and the plasma membrane (PM) form ER-PM contact sites (EPCSs) that allow the ER and PM to exchange materials and information. Stress-induced disruption of protein folding triggers ER stress, and the cell initiates the unfolded protein response (UPR) to resist the stress. However, whether EPCSs play a role in ER stress in plants remains unclear. VESICLE-ASSOCIATED MEMBRANE PROTEIN (VAMP)-ASSOCIATED PROTEIN 27-1 (VAP27-1) functions in EPCS tethering and is encoded by a family of 10 genes (VAP27-1-10) in Arabidopsis thaliana. Here, we used CRISPR-Cas9-mediated genome editing to obtain a homozygous vap27-1 vap27-3 vap27-4 (vap27-1/3/4) triple mutant lacking three of the key VAP27 family members in Arabidopsis. The vap27-1/3/4 mutant exhibits defects in ER-PM connectivity and EPCS architecture, as well as excessive UPR signaling. We further showed that relocation of VAP27-1 to the PM mediates specific VAP27-1-related EPCS remodeling and expansion under ER stress. Moreover, the spatiotemporal dynamics of VAP27-1 at the PM increase ER-PM connectivity and enhance Arabidopsis resistance to ER stress. In addition, we revealed an important role for intracellular calcium homeostasis in the regulation of UPR signaling. Taken together, these results broaden our understanding of the molecular and cellular mechanisms of ER stress and UPR signaling in plants, providing additional clues for improving plant broad-spectrum resistance to different stresses.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11287176 | PMC |
| http://dx.doi.org/10.1016/j.xplc.2024.100929 | DOI Listing |
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