AI Article Synopsis
- Citri Reticulatae Pericarpium is a traditional Chinese medicine known for its high health benefits and clinical value, primarily due to its active secondary metabolites, which are flavonoids.
- A new method was developed to comprehensively analyze these flavonoids using a targeted MS/MS approach, which involved creating a database of known flavonoids and locking onto their precursor ions.
- Through this analysis, a total of 187 flavonoids were identified from the Citri Reticulatae Pericarpium, including some chalcones that had not been characterized before, expanding the understanding of its chemical composition.
Article Abstract
Citri Reticulatae Pericarpium is a traditional Chinese medicine with extremely high health benefits as well as clinical value. In vivo and in vitro tests have proved that its main active secondary metabolites are flavonoids. However, they have not been comprehensively analyzed up to now mainly due to lack of suitable analysis method. To solve this problem, a novel strategy based on precursor ions locked and targeted MS/MS analysis was proposed. Firstly, the database of the flavonoids previously isolated from Citri Reticulatae Pericarpium was established to obtain the characteristics of their precursor ions. Secondly, after performing the full MS scan of the extract, all compounds in the total ion chromatogram were extracted by Compound Discoverer software. Thirdly, the precursor ions of the flavonoids were locked from the extracted compounds according to their characteristics, forming a precursor ions list. Finally, the precursor ions in the constructed list were performed targeted MS/MS analysis for structures characterization. As a result, total 187 flavonoids were successfully identified, and except for flavones, flavonols as well as dihydroflavones, some chalcones were also characterized from Citri Reticulatae Pericarpium for the first time.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11055944 | PMC |
| http://dx.doi.org/10.1038/s41598-024-60415-w | DOI Listing |
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