Experimental Susceptibility of and (Psychodidae: Phlebotominae) to () and () (Trypanosomatidae: Leishmaniinae).

Microorganisms

Programa de Pós Graduação em Biologia de Agentes Infecciosos e Parasitários, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém 66075-110, PA, Brazil.

Published: April 2024

The present work assessed the experimental susceptibility of and to () and (.) . A () - combination was used as a susceptible control. Wild-caught and laboratory-bred were membrane-fed on blood with a 5 × 10/mL log-phase promastigote culture suspension and dissected on days 2 and 8 post-blood meal (pbm) for analysis focused on the assessment of parasitoses, as well as placement and promastigote morphotyping. Survival curves were constructed. In all combinations, promastigotes were observed on day 8 pbm. For both species, in , the presence of parasites was observed up to the stomodeal valve, while in , the presence of parasites was observed up to the cardia. There were no significant differences in parasitosis between () and () in either or . Six morphological promastigote forms were distinguished in Giemsa-stained gut smears. The survival curves of all combinations decreased and were affected differently by several -parasite combinations, as well with -uninfected blood. These findings stress as experimentally susceptible to spp. and suggest the putative susceptibility of to () and () .

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11051748PMC
http://dx.doi.org/10.3390/microorganisms12040809DOI Listing

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