AI Article Synopsis
- The study focused on identifying contamination by endospore-forming aerobic bacteria in a pharmaceutical production facility in Rio de Janeiro, emphasizing the importance of accurate species identification for preventing contamination.
- Three methods were used for characterization: MALDI-TOF MS with the MALDI Biotyper and VITEK MS systems, as well as complete 16S rRNA gene sequencing.
- Results revealed several prevalent bacterial strains, with some showing less than 98.7% DNA similarity to known species, suggesting potential new species, while highlighting the need for additional analyses beyond the initial methods for complete identification.
Article Abstract
and related genera are among the most important contaminants in the pharmaceutical production environment, and the identification of these microorganisms at the species level assists in the investigation of sources of contamination and in preventive and corrective decision making. The aim of this study was to evaluate three methodologies for the characterization of endospore-forming aerobic bacterial strains isolated from a pharmaceutical unit in Rio de Janeiro, Brazil. MALDI-TOF MS was performed using MALDI Biotyper and VITEK MS RUO systems, and complete 16S rRNA gene sequencing was performed using the Sanger methodology. The results showed the prevalence of the genera (n = 9; 36.0%), (n = 5; 20.0%), and (n = 4; 16.0%). Three (20.0%) strains showed <98.7% of DNA sequencing similarity on the EzBioCloud Database, indicating possible new species. In addition, the reclassification of to the genus as sp. nov. is proposed. In conclusion, 16S rRNA and MALDI TOF/MS were not sufficient to identify all strains at the species level, and complementary analyses were necessary.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11051829 | PMC |
| http://dx.doi.org/10.3390/microorganisms12040724 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Characteristics of gut microbiota profiles in Asian elephants (Elephas maximus) with gastrointestinal disorders.
Sci Rep
January 2025
Center of Elephant and Wildlife Health, Animal Hospital, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand.
Colic and diarrhea are common gastrointestinal (GI) disorders in captive Asian elephants, which can severely impact health and lead to mortality. Gut dysbiosis, indicated by alterations in gut microbiome composition, can be observed in individuals with GI disorders. However, changes in gut microbial profiles of elephants with GI disorders have never been investigated.
View Article and Find Full Text PDFInfluence of selected dosages of plastic microparticles on the porcine fecal microbiome.
Sci Rep
January 2025
Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719, Olsztyn, Poland.
Studies conducted so far have shown that nano- and microplastic may disturb the intestinal microenvironment by interacting with the intestinal epithelium and the gut microbiota. Depending on the research model used, the effect on the microbiome is different-an increase or decrease in selected taxa resulting in the development of dysbiosis. Dysbiosis may be associated with intestinal inflammation, development of mental disorders or diabetes.
View Article and Find Full Text PDFAlgal-bacterial bioremediation of cyanide-containing wastewater in a continuous stirred photobioreactor.
World J Microbiol Biotechnol
January 2025
The Biotechnology Center, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt.
This study reports the isolation and characterization of highly resistant bacterial and microalgal strains from an Egyptian wastewater treatment station to cyanide-containing compounds. The bacterial strain was identified as Bacillus licheniformis by 16S rRNA gene sequencing. The isolate removed up to 1 g L potassium cyanide, 3 g L benzonitrile, and 1 g L sodium salicylate when incubated as 10% v/v in MSM at 30 ℃.
View Article and Find Full Text PDFExploration of Cytokines and Microbiome Among Males and Females with Diarrhea-Predominant Irritable Bowel Syndrome.
Dig Dis Sci
January 2025
School of Nursing, University of Washington, Seattle, WA, USA.
Background: Whether pathophysiological factors differ between males and females with irritable bowel syndrome-diarrhea (IBS-D) remains to be tested. To better understand potential sex differences, males with IBS-D were compared to naturally cycling females and to females with IBS-D taking hormonal contraception on plasma levels of cytokines and gut microbiome characteristics.
Methods: Males and females with Rome III IBS-D completed questionnaires and kept a daily symptom diary for 28 days.
New Strains of the Deep Branching Streptophyte Streptofilum: Phylogenetic Position, Cell Biological and Ecophysiological Traits, and Description of Streptofilum arcticum sp. nov.
Environ Microbiol
January 2025
Institute for Biological Sciences, Applied Ecology and Phycology, University Rostock, Rostock, Germany.
Streptofilum capillatum was recently described and immediately caught scientific attention, because it forms a phylogenetically deep branch in the streptophytes and is characterised by a unique cell coverage composed of piliform scales. Its phylogenetic position and taxonomic rank are still controversial discussed. In the present study, we isolated further strains of Streptofilum from biocrusts in sand dunes and Arctic tundra soil.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!