and Expression Contributes to COVID-19 Disease Severity within a South African Cohort.

Globally, SARS-CoV-2 has negatively impacted many lives and industries due to its rapid spread, severe outcomes, and the need for the implementation of lockdown strategies across the world. SARS-CoV-2 disease severity varies among different populations. Host genetics have been associated with various diseases, and their ability to alter disease susceptibility and severity. In addition, Human Leukocyte Antigen () expression levels and alleles vary significantly among ethnic groups, which might impact the host's response to SARS-CoV-2. Our previous study highlighted that might have an effect on COVID-19 disease severity across ethnicities. Therefore, in this study, we aim to examine the effect of and expression levels on COVID-19 disease severity. To achieve this, we used real-time PCR to measure the mRNA expression levels of SARS-CoV-2-infected individuals from a South African cohort and compared them across ethnic groups, disease outcomes, gender, comorbidities, and age. Our results show (1) that the effect of mRNA expression levels was associated with differences in disease severity when we compare symptomatic vs. asymptomatic ( < 0.0001). While mRNA expression levels were not associated with COVID-19 disease severity. (2) In addition, we observed that and mRNA expression levels were significantly different between South African Black individuals and South African Indian individuals ( < 0.0001, < 0.0001). mRNA expression levels among symptomatic South African Black individuals were significantly higher than symptomatic South African Indian individuals ( < 0.0001). In addition, the mRNA expression levels of symptomatic South African Black individuals were significantly higher than asymptomatic South African Black individuals ( > 0.0001). mRNA expression levels among symptomatic South African Black individuals were significantly higher than among symptomatic South African Indian individuals ( = 0.0217). (3) expression levels were significantly different between males and females ( = 0.0052). In addition, the expression levels of asymptomatic males are higher than asymptomatic females ( = 0.0375). (4) expression levels were significantly different between individuals with and without comorbidities ( = 0.0009). In addition, we observed a significant difference between individuals with no comorbidities and non-communicable diseases ( = 0.0034), in particular, hypertension ( = 0.0487). (5) expression levels were significantly different between individuals between 26-35 and 56-65 years ( = 0.0380). Our work is expected to strengthen the understanding of the relationship between and COVID-19 by providing insights into and expression levels across ethnic populations in South Africa among COVID-19-symptomatic and asymptomatic individuals. Our results highlight that mRNA expression levels contribute to COVID-19 severity as well as variation in ethnicities associated with COVID-19. Further studies are needed to examine the effect of expression levels across various ethnic groups with contributing factors.