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Systems Metabolic Engineering for Efficient Violaxanthin Production in Yeast. | LitMetric

Systems Metabolic Engineering for Efficient Violaxanthin Production in Yeast.

J Agric Food Chem

Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Life Sciences, Faculty of Medicine, Tianjin University, Tianjin 300072, China.

Published: May 2024

AI Article Synopsis

  • Violaxanthin is a powerful antioxidant found in plants, important in industries like food, agriculture, and cosmetics, and serves as a precursor for substances like abscisic acid and fucoxanthin.
  • Researchers implemented a comprehensive strategy using zeaxanthin epoxidase screening, redox state engineering, and NADPH regeneration to significantly boost violaxanthin production, achieving record levels.
  • This study establishes a more efficient method for producing violaxanthin and zeaxanthin, providing valuable insights for future microbial production of xanthophylls.

Article Abstract

Violaxanthin is a plant-derived orange xanthophyll with remarkable antioxidant activity that has wide applications in various industries, such as food, agriculture, and cosmetics. In addition, it is the key precursor of important substances such as abscisic acid and fucoxanthin. , as a GRAS (generally regarded as safe) chassis, provides a good platform for producing violaxanthin production with a yield of 7.3 mg/g DCW, which is far away from commercialization. Herein, an integrated strategy involving zeaxanthin epoxidase (ZEP) source screening, cytosol redox state engineering, and nicotinamide adenine dinucleotide phosphate (NADPH) regeneration was implemented to enhance violaxanthin production in . 58aa-truncated from exhibited optimal efficiency in an efficient zeaxanthin-producing strain. The titer of violaxanthin gradually increased by 17.9-fold (up to 119.2 mg/L, 15.19 mg/g DCW) cytosol redox state engineering and NADPH supplementation. Furthermore, balancing redox homeostasis considerably improved the zeaxanthin concentration by 139.3% (up to 143.9 mg/L, 22.06 mg/g DCW). Thus, the highest reported titers of violaxanthin and zeaxanthin in were eventually achieved. This study not only builds an efficient platform for violaxanthin biosynthesis but also serves as a useful reference for the microbial production of xanthophylls.

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Source
http://dx.doi.org/10.1021/acs.jafc.4c01240DOI Listing

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