AI Article Synopsis
- - The study focuses on vascular smooth muscle cells (VSMCs) and aims to improve their use in tissue-engineered vascular constructions by utilizing a three-dimensional culture system.
- - Researchers treated calf VSMCs with tumor necrosis factor-alpha (TNF-α) before culturing them in different settings, and then assessed their characteristics using various scientific methods.
- - Results showed that TNF-α caused VSMCs to switch from a contractile to a synthetic phenotype, significantly enhancing their proliferation and migration abilities in both two-dimensional and three-dimensional cultures.
Article Abstract
Background: Vascular smooth muscle cells (VSMCs) are commonly used as seed cells in tissue-engineered vascular constructions. However, their variable phenotypes and difficult to control functions pose challenges. This study aimed to overcome these obstacles using a three-dimensional culture system.
Methods: Calf VSMCs were administered tumor necrosis factor-alpha (TNF-α) before culturing in two- and three-dimensional well plates and polyglycolic acid (PGA) scaffolds, respectively. The phenotypic markers of VSMCs were detected by immunofluorescence staining and western blotting, and the proliferation and migration abilities of VSMCs were detected by CCK-8, EDU, cell counting, scratch, and Transwell assays.
Results: TNF-α rapidly decreased the contractile phenotypic markers and elevated the synthetic phenotypic markers of VSMCs, as well as markedly increasing the proliferation and migration ability of VSMCs under two- and three-dimensional culture conditions.
Conclusions: TNF-α can rapidly induce a phenotypic shift in VSMCs and change their viability on PGA scaffolds.
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| http://dx.doi.org/10.1111/aor.14762 | DOI Listing |
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