AI Article Synopsis
- Enterobacter cloacae complex (ECC) is a significant pathogen in hospitals, prompting research on its distribution and resistance to antibiotics, particularly carbapenems.
- A study collected 70 ECC isolates and found that 20 were positive for class 1 integron integrase gene (intI1), indicating a notable resistance to common antimicrobial agents compared to intI1-negative isolates.
- The research identified 19 antimicrobial-resistant gene cassettes and found the sequence of the carbapenem-resistant gene bla in an Enterobacter hormaechei isolate, marking the first report of this specific gene in ECC in China.
Article Abstract
Enterobacter cloacae complex (ECC) widely exists in the hospital environment and is one of the important conditional pathogens of hospital-acquired infection. To investigate the distribution of integrons and carbapenem-resistant genes in clinical ECC, 70 isolates of ECC from non-sputum specimens were collected. Class 1 and class 2 integron integrase gene intI1 and intI2, as well as common carbapenem-resistant genes, bla, bla, bla, bla, bla and bla, were screened. Gene cassette arrays and common promoters of class 1 integron together with subtypes of carbapenem-resistant genes were determined by sequencing. Resistant rates to commonly used antimicrobial agents between class 1 integron-positive and integron-negative ECC isolates were analyzed. The whole-genome of bla harboring Enterobacter hormaechei was sequenced and the sequence around bla was analyzed. Twenty isolates were positive for intI1. Nineteen different antimicrobial-resistant gene cassettes and 11 different gene cassette arrays, including aadA22-lnuF, were detected in this study. Common promoters of class 1 integron PcH1, PcW, PcW-P2, and PcH2 were detected in 12, 4, 3, and 1 isolates, respectively. The rates of antimicrobial resistance of intI1-positive isolates were higher than those of intI1-negative isolates to clinical commonly used antimicrobial agents. Carbapenem-resistant genes bla, bla, bla and bla were detected in 2, 1, 1, and 1 isolates, respectively. bla was located between ble and IS5. To the best of our knowledge, this study reported for the first time of bla in ECC isolate in China.
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| http://dx.doi.org/10.1007/s00284-024-03679-9 | DOI Listing |
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