[Identification and expression pattern analysis of a secretory phospholipase gene in ].

Phospholipase A2 (PLA2) is widely distributed in animals, plants, and microorganisms, and it plays an important role in many physiological activities. In a previous study, we have identified a secretory PLA2 in (). In this study, we further identified four new genes (, , , and ) in . genome. All four genes exhibits the characteristic features of sPLA2, including the sPLA2 domain, metal binding sites, and highly conserved catalytic domain. This study completed the cloning, expression, and expression pattern analysis of the gene in . . The full length of is 585 bp, and the recombinant protein obtained through prokaryotic expression has an estimated size of 25 kDa. qRT-PCR analysis revealed that the expression level of reached its peak on the first day of the fifth instar larval stage. Tissue expression profiling analysis showed that had the highest expression level in the midgut, followed by the epidermis and fat body. Western blotting analysis results were consistent with those of qRT-PCR. Furthermore, after infecting fifth instar 1-day-old larvae with and , the expression level of the gene significantly increased in 24 h. The findings of this study provides a theoretical basis and valuable experimental data for future related research.